Comparative transcriptome expression analysis in susceptible and resistant potato (Solanum tuberosum) cultivars to common scab (Streptomyces scabies) revealed immune priming responses in the incompatible interaction

PloS One
Bourlaye FofanaKaushik Ghose

Abstract

Common scab disease in potato has become a widespread issue in major potato production areas, leading to increasing economic losses. Varietal resistance is seen as a viable and long-term scab management strategy. However, the genes and mechanisms of varietal resistance are unknown. In the current study, a comparative RNA transcriptome sequencing and differential gene signaling and priming sensitization studies were conducted in two potato cultivars that differ by their response to common scab (Streptomyces scabies), for unraveling the genes and pathways potentially involved in resistance within this pathosystem. We report on a consistent and contrasted gene expression pattern from 1,064 annotated genes differentiating a resistant (Hindenburg) and a susceptible (Green Mountain) cultivars, and identified a set of 273 co-regulated differentially expressed genes in 34 pathways that more likely reflect the genetic differences of the cultivars and metabolic mechanisms involved in the scab pathogenesis and resistance. The data suggest that comparative transcriptomic phenotyping can be used to predict scab lesion phenotype in breeding lines using mature potato tuber. The study also showed that the resistant cultivar, Hindenburg, has de...Continue Reading

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Citations

Mar 18, 2021·Plant Disease·Guilherme da Silva PereiraG Craig Yencho

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Datasets Mentioned

BETA
PRJNA532699
GM

Methods Mentioned

BETA
RNAseq
PCA
gene knockout

Software Mentioned

David
Bowtie2
R3
R
GenStat
Cufflinks
FASQC
Tophat
Cuffdiff
D atabase for A nnotation , V isualization and I ntegrated D i...

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