Comparative Transcriptomics Analysis of the Responses of the Filamentous Fungus Glarea lozoyensis to Different Carbon Sources

Frontiers in Microbiology
Ke ZhangYuwen Wang

Abstract

The natural product pneumocandin B0 is the precursor of the antifungal drug caspofungin. We found that replacing glucose in the initial fermentation medium with 20 g/L fructose is more conducive to pneumocandin B0 production and biomass accumulation. In order to explore the mechanism of the different metabolic responses to fructose and glucose, we used each as the sole carbon source, and the results showed that fructose increased the total pneumocandin B0 yield and biomass by 54.76 and 13.71%, respectively. Furthermore, we analyzed the differences of gene expression and metabolic pathways between the two different carbon sources by transcriptomic analysis. When fructose was used as the carbon source, genes related to the pentose phosphate pathway (PPP), glycolysis and branched-chain amino acid metabolism were significantly upregulated, resulting in increased intracellular pools of NADPH and acetyl-CoA in Glarea lozoyensis for cell growth and pneumocandin B0 product synthesis. Interestingly, the pneumocandin B0 biosynthetic gene cluster and the genes of the TCA cycle were significantly downregulated, while the FAS genes were significantly upregulated, indicating that more acetyl-CoA was used for fatty acid synthesis. In particul...Continue Reading

References

Apr 1, 1977·The Journal of Antibiotics·K MizunoM Hayashi
Feb 1, 1989·The Journal of Antibiotics·C F WichmannR E Schwartz
May 26, 2001·Annual Review of Nutrition·P J Trotter
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Mar 21, 2009·Amino Acids·Guoyao Wu
Mar 10, 2010·Critical Reviews in Microbiology·Beatriz RuizElizabeth Langley
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Nov 12, 2010·Trends in Endocrinology and Metabolism : TEM·Varman T Samuel
Dec 15, 2010·Current Protocols in Bioinformatics·Ben Langmead
Dec 24, 2010·Genome Biology·Alicia OshlackMatthew D Young
May 24, 2014·Metabolic Engineering·Jiazhang LianHuimin Zhao
Jan 8, 2015·Applied Biochemistry and Biotechnology·Tinglan LiQiang Hua
Jun 4, 2015·Cell Metabolism·Federico PietrocolaGuido Kroemer
Oct 13, 2016·Annual Review of Genetics·Juliane MacheleidtAxel A Brakhage
Dec 26, 2016·Metabolic Engineering·Gao-Yi Tan, Tiangang Liu
Aug 9, 2017·Science China. Life Sciences·Yue Li, Huarong Tan
Oct 8, 2017·Biotechnology and Bioengineering·Macarena LarroudeRodrigo Ledesma-Amaro
Jun 28, 2018·Journal of Industrial Microbiology & Biotechnology·Ping SongHe Huang
Nov 1, 2016·Bioscience, Biotechnology, and Biochemistry·Tingting QinHe Huang

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP220084

Methods Mentioned

BETA
PCR
RNA-seq
Illumina sequencing

Software Mentioned

Blast2 GO
BLAST
bowtie2
Tophat2
BLASTX
Perl script
DESeq
Cufflinks

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.