Comparative transcriptomics of multidrug-resistant Acinetobacter baumannii in response to antibiotic treatments

Scientific Reports
Hao QinTing-Fung Chan

Abstract

Multidrug-resistant Acinetobacter baumannii, a major hospital-acquired pathogen, is a serious health threat and poses a great challenge to healthcare providers. Although there have been many genomic studies on the evolution and antibiotic resistance of this species, there have been very limited transcriptome studies on its responses to antibiotics. We conducted a comparative transcriptomic study on 12 strains with different growth rates and antibiotic resistance profiles, including 3 fast-growing pan-drug-resistant strains, under separate treatment with 3 antibiotics, namely amikacin, imipenem, and meropenem. We performed deep sequencing using a strand-specific RNA-sequencing protocol, and used de novo transcriptome assembly to analyze gene expression in the form of polycistronic transcripts. Our results indicated that genes associated with transposable elements generally showed higher levels of expression under antibiotic-treated conditions, and many of these transposon-associated genes have previously been linked to drug resistance. Using co-expressed transposon genes as markers, we further identified and experimentally validated two novel genes of which overexpression conferred significant increases in amikacin resistance. T...Continue Reading

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Citations

Mar 20, 2019·Nature Reviews. Genetics·Manish BoolchandaniGautam Dantas
Feb 21, 2019·The Journal of Antimicrobial Chemotherapy·Wuen Ee FoongKlaas M Pos
Mar 20, 2019·Expert Opinion on Drug Discovery·Vladislav M ChernovRustam I Aminov
Nov 16, 2019·NPJ Biofilms and Microbiomes·Anahit PenesyanIan T Paulsen
Nov 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Thomas R AuninsAnushree Chatterjee
Feb 28, 2021·Scientific Reports·Jasmine MartinezMaria Soledad Ramirez
Nov 6, 2020·NPJ Biofilms and Microbiomes·Anahit PenesyanIan T Paulsen
Jun 3, 2021·Antibiotics·Diego Lucas Neres RodriguesFlávia Figueira Aburjaile
Jun 10, 2021·Human Vaccines & Immunotherapeutics·NorAziyah Mat RahimSazaly AbuBakar

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Datasets Mentioned

BETA
SRP075337

Methods Mentioned

BETA
electrophoresis
2′-phosphotransferase
Reverse-transcription PCR
PCR
RNA-seq
protein folding

Software Mentioned

MEGA
Trimmomatic
BLAST
BLASTX
MUSCLE
Blast2GO
Trinity

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