Comparative transcripts profiling reveals new insight into molecular processes regulating lycopene accumulation in a sweet orange (Citrus sinensis) red-flesh mutant.

BMC Genomics
Qiang XuXiuxin Deng

Abstract

Interest in lycopene metabolism and regulation is growing rapidly because accumulative studies have suggested an important role for lycopene in human health promotion. However, little is known about the molecular processes regulating lycopene accumulation in fruits other than tomato so far. On a spontaneous sweet orange bud mutant with abnormal lycopene accumulation in fruits and its wild type, comparative transcripts profiling was performed using Massively Parallel Signature Sequencing (MPSS). A total of 6,877,027 and 6,275,309 reliable signatures were obtained for the wild type (WT) and the mutant (MT), respectively. Interpretation of the MPSS signatures revealed that the total number of transcribed gene in MT is 18,106, larger than that in WT 17,670, suggesting that newly initiated transcription occurs in the MT. Further comparison of the transcripts abundance between MT and WT revealed that 3,738 genes show more than two fold expression difference, and 582 genes are up- or down-regulated at 0.05% significance level by more than three fold difference. Functional assignments of the differentially expressed genes indicated that 26 reliable metabolic pathways are altered in the mutant; the most noticeable ones are carotenoid bi...Continue Reading

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Citations

Dec 14, 2011·Briefings in Functional Genomics·Mukesh Jain
Jun 22, 2013·Physiologia Plantarum·Yunliu ZengXiuxin Deng
Mar 11, 2015·Genome Génome / Conseil National De Recherches Canada·Chunxian ChenFred G Gmitter
Jul 25, 2018·Horticulture Research·Toshi M Foster, Maria José Aranzana
Jul 14, 2021·Journal of Agricultural and Food Chemistry·Cuihua LiuJuan Xu

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Methods Mentioned

BETA
ubiquitination
PCR

Software Mentioned

SPSS
LongSAGE
MPSS
KEGG
Annot8r
BLAST
Primer Express
Sequence Detector

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