Comparative Venomics Reveals the Complex Prey Capture Strategy of the Piscivorous Cone Snail Conus catus

Journal of Proteome Research
S W A HimayaRichard J Lewis

Abstract

Venomous marine cone snails produce a unique and remarkably diverse range of venom peptides (conotoxins and conopeptides) that have proven to be invaluable as pharmacological probes and leads to new therapies. Conus catus is a hook-and-line fish hunter from clade I, with ∼20 conotoxins identified, including the analgesic ω-conotoxin CVID (AM336). The current study unravels the venom composition of C. catus with tandem mass spectrometry and 454 sequencing data. From the venom gland transcriptome, 104 precursors were recovered from 11 superfamilies, with superfamily A (especially κA-) conotoxins dominating (77%) their venom. Proteomic analysis confirmed that κA-conotoxins dominated the predation-evoked milked venom of each of six C. catus analyzed and revealed remarkable intraspecific variation in both the intensity and type of conotoxins. High-throughput FLIPR assays revealed that the predation-evoked venom contained a range of conotoxins targeting the nAChR, Cav, and Nav ion channels, consistent with α- and ω-conotoxins being used for predation by C. catus. However, the κA-conotoxins did not act at these targets but induced potent and rapid immobilization followed by bursts of activity and finally paralysis when injected intram...Continue Reading

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Nov 29, 2015·Molecular Ecology·J R PrashanthR J Lewis
Jun 19, 2014·PloS One·Cecilia A PratorJoseph R Schulz
Mar 10, 2018·International Journal of Molecular Sciences·S W A Himaya, Richard J Lewis
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Nov 2, 2017·Molecular BioSystems·Jutty Rajan PrashanthRichard James Lewis
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Jul 28, 2019·Marine Drugs·Santos Ramírez-CarretoClaudia Rodríguez-Almazán
Mar 15, 2020·The Protein Journal·Manyi Yang, Maojun Zhou
Jun 26, 2021·Scientific Reports·S W A HimayaRichard J Lewis
Oct 22, 2019·Chemical Reviews·Ai-Hua JinPaul F Alewood

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