Comparative whole genome transcriptome analysis and fenugreek leaf extract modulation on cadmium‑induced toxicity in liver cells

International Journal of Molecular Medicine
Caroline O OdewumiMarijo Kent-First

Abstract

Cadmium (Cd), an economically valuable metal, is widely used in various industrial processes. Although it is of economic value, it is hazardous to human health. Cd accumulates in vital organs where it causes various diseases. Natural compounds with chelating or antioxidant properties have been tested to reduce the toxic effect of Cd. The anti‑oxidant, anti‑diabetic and hypocholesterolemic properties of fenugreek (Trigonella foenum-graecum) leaves make it a candidate for investigation as protective agent against Cd‑induced toxicity. In the present study, the protective effects of fenugreek leaf extract (FLE) on cell viability, morphology, and whole genomic transcription in cadmium chloride (CdCl2)‑treated rat liver cells were analyzed. The cells were treated with 25 µM CdCl2 alone, or co‑treated with 5 µg/ml FLE for 48 h. The co‑treated cells were pretreated with FLE for 2 or 4 h, followed by CdCl2 treatment. Genomic transcription analysis was performed in the CdCl2‑treated cells following treatment for 6 h. The CdCl2 caused a significant decrease in viability (35.8±4.1%) and morphological distortion of the cells, compared with the untreated control cells; whereas 4 h pretreatment with FLE (5 µg/ml) reversed the Cd‑induced morph...Continue Reading

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Methods Mentioned

BETA
PCR

Software Mentioned

Ariadne
Pathway Studio
Partek Express
GeneAtlas

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