Comparing Effects of Transforming Growth Factor β1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels

Frontiers in Cellular Neuroscience
Starlee LivelyLyanne C Schlichter

Abstract

The cytokine, transforming growth factor β1 (TGFβ1), is up-regulated after central nervous system (CNS) injuries or diseases involving microglial activation, and it has been proposed as a therapeutic agent for treating neuroinflammation. Microglia can produce and respond to TGFβ1. While rats and mice are commonly used for studying neuroinflammation, very few reports directly compare them. Such studies are important for improving pre-clinical studies and furthering translational progress in developing therapeutic interventions. After intracerebral hemorrhage (ICH) in the rat striatum, the TGFβ1 receptor was highly expressed on microglia/macrophages within the hematoma. We recently found species similarities and differences in response to either a pro-inflammatory (interferon-γ, IFN-γ, +tumor necrosis factor, TNF-α) or anti-inflammatory interleukin-4 (IL-4) stimulus. Here, we assessed whether rat and mouse microglia differ in their responses to TGFβ1. Microglia were isolated from Sprague-Dawley rats and C57BL/6 mice and treated with TGFβ1. We quantified changes in expression of >50 genes, in their morphology, proliferation, apoptosis and in three potassium channels that are considered therapeutic targets. Many inflammatory mediat...Continue Reading

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Citations

Aug 9, 2018·Frontiers in Cellular Neuroscience·Starlee Lively, Lyanne C Schlichter
Oct 28, 2019·Frontiers in Cellular Neuroscience·Björn LafferMaren Kasper
Dec 14, 2018·Frontiers in Cellular Neuroscience·Starlee LivelyLyanne C Schlichter
Mar 21, 2020·Journal of Neuroinflammation·Miriam MechaCarmen Guaza
Apr 24, 2021·Brain and Neuroscience Advances·Jonathan Davis, Erik Mire
May 10, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·David A HumeKatharine M Irvine
Jun 11, 2021·The European Journal of Neuroscience·Tomohiro OhgomoriShozo Jinno

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Methods Mentioned

BETA
transgenic
protein assay

Key Resources (RRID) Mentioned

SCR_013553
AB_321301
SCR_012931
AB_778352
AB_2340846
AB_2340621
SCR_008988
SCR_013530
SCR_003070
SCR_008452

Software Mentioned

pCLAMP
R
R Project for Statistical Computing
nSolver Analysis
GraphPad Prism
Nanostring
ImageJ
Image Lab

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