Comparing Electron Leak in Vertebrate Muscle Mitochondria

Integrative and Comparative Biology
Jason R TrebergLilian Wiens

Abstract

Mitochondrial electron transfer for oxidative ATP regeneration is linked to reactive oxygen species (ROS) production in aerobic eukaryotic cells. Because they can contribute to signaling as well as oxidative damage in cells, these ROS have profound impact for the physiology and survival of the organism. Although mitochondria have been recognized as a potential source for ROS for about 50 years, the mechanistic understanding on molecular sites and processes has advanced recently. Most experimental approaches neglect thermal variability among species although temperature impacts mitochondrial processes significantly. Here we delineate the importance of temperature by comparing muscle mitochondrial ROS formation across species. Measuring the thermal sensitivity of respiration, electron leak rate (ROS formation), and the antioxidant capacity (measured as H2O2 consumption) in intact mitochondria of representative ectothermic and endothermic vertebrate species, our results suggest that using a common assay temperature is inappropriate for comparisons of organisms with differing body temperatures. Moreover, we propose that measuring electron leak relative to the mitochondrial antioxidant capacity (the oxidant ratio) may be superior to...Continue Reading

References

Apr 1, 1971·The American Journal of Physiology·G A BrooksR E Beyer
Jul 1, 1972·The Biochemical Journal·A BoverisB Chance
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Nadir Askenasy, Alan P Koretsky
Oct 3, 2002·Journal of Neurochemistry·Anatoly A StarkovGary Fiskum
Aug 13, 2003·Journal of Neurochemistry·Anatoly A Starkov, Gary Fiskum
Aug 30, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S C LearyC D Moyes
Nov 25, 2003·The Journal of Biological Chemistry·Franco ZoccaratoAdolfo Alexandre
May 12, 2004·Respiratory Physiology & Neurobiology·Ewald R WeibelHans Hoppeler
Nov 17, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Grant B McClelland
Apr 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kevin R ShortK Sreekumaran Nair
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·A Yu AndreyevA A Starkov
Oct 28, 2005·American Journal of Physiology. Cell Physiology·Ethan J Anderson, P Darrell Neufer
Jan 13, 2006·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A J HulbertHelga Guderley
Jun 19, 2010·The Journal of Biological Chemistry·Derek A Drechsel, Manisha Patel
Jun 29, 2011·The Journal of Biological Chemistry·Casey L QuinlanMartin D Brand
Oct 14, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jason C L BrownJames F Staples
Nov 8, 2011·Methods in Molecular Biology·Charles AffourtitMartin D Brand
Jun 13, 2012·The Journal of Biological Chemistry·Casey L QuinlanMartin D Brand
May 23, 2014·Current Biology : CB·Michael Schieber, Navdeep S Chandel
Sep 25, 2014·Journal of Bioenergetics and Biomembranes·Anatoly A StarkovVasily N Popov
Oct 31, 2014·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Imen Belhadj SlimenManef Abdrabbah
Oct 13, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sheena BanhJason R Treberg
Mar 31, 2017·The Journal of Experimental Biology·Daniel Munro, Jason R Treberg
Dec 19, 2017·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jason R TrebergKathryn Kroeker

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Citations

Jun 4, 2019·Cell Cycle·Mehrdad GhashghaeiniaIngolf Bernhardt
Sep 22, 2018·Integrative and Comparative Biology·Wendy R HoodKarine Salin
Jan 19, 2021·Oxidative Medicine and Cellular Longevity·Iwona KwiatkowskaDariusz Pawlak
Jan 14, 2021·Trends in Ecology & Evolution·Rebecca E KochAntoine Stier
May 10, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jason R Treberg
Aug 22, 2021·Antioxidants & Redox Signaling·Eftekhar Eftekharpour, Paul Fernyhough

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