Comparing Gut Microbiome in Mothers' Own Breast Milk- and Formula-Fed Moderate-Late Preterm Infants

Frontiers in Microbiology
Ziyi WangShin-Yi Lee Marzano

Abstract

Gut microbiome plays an important role in adult human health and diseases. However, how nutritional factors shape the initial colonization of gut bacteria in infants, especially in preterm infants, is still not completely known. In this study, we compared the effects of feeding with mothers' own breast milk (MBM) and formula on the initial composition and gene expression of gut bacteria in moderate-late preterm infants. Fecal samples were collected from ten formula-fed and ten MBM healthy infants born between 32 and 37 weeks' gestation after they reached full-volume enteral feedings. Total DNAs were extracted from fecal samples for amplicon sequencing of 16S ribosomal RNA (rRNA) gene and total RNA with rRNA depletion for metatranscriptome RNA-Seq 16S rRNA gene amplicon sequencing results showed that the alpha-diversity was similar between the MBM- and formula-fed preterm infants, but the beta-diversity showed a significant difference in composition (p = 0.002). The most abundant taxa were Veillonella (18.4%) and Escherichia/Shigella (15.2%) in MBM infants, whereas the most abundant taxa of formula-fed infants were Streptococcus (18.6%) and Klebsiella (17.4%). The genera Propionibacterium, Streptococcus, and Finegoldia and order...Continue Reading

References

Aug 1, 1976·Journal of Medical Microbiology·C L BullenA T Willis
Sep 1, 1978·Acta paediatrica Scandinavica·L A HansonC S Edén
Nov 1, 1992·Diabetic Medicine : a Journal of the British Diabetic Association·S M VirtanenH K Akerblom
Dec 22, 1990·Lancet·A Lucas, T J Cole
Dec 1, 1989·Archives of Disease in Childhood·S E Balmer, B A Wharton
Oct 1, 1983·Scandinavian Journal of Gastroenterology·O Bergstrand, G Hellers
Jan 1, 1994·Antonie van Leeuwenhoek·J R Andreesen
Apr 22, 1999·Archives of Disease in Childhood. Fetal and Neonatal Edition·I H GewolbP Panigrahi
May 8, 1999·The American Journal of Clinical Nutrition·R I MackieH R Gaskins
May 8, 1999·The American Journal of Clinical Nutrition·M D Collins, G R Gibson
Feb 16, 2000·Pharmacology & Therapeutics·J Y Chou
Mar 21, 2000·American Journal of Human Genetics·M E ChamberlinJ Y Chou
Jun 6, 2000·The American Journal of Clinical Nutrition·M B EngferH Daniel
Dec 9, 2000·The Journal of Nutrition·M J GnothS Rudloff
Feb 7, 2001·The American Journal of Clinical Nutrition·W H HolzapfelU Schillinger
Mar 10, 2001·Pediatric Clinics of North America·M Hamosh
Jun 22, 2002·Lancet·Julie ArmstrongUNKNOWN Child Health Information Team
May 27, 2003·Molecular Genetics and Metabolism·S Harvey MuddMarlene W Borschel
Oct 1, 1961·Journal of Bacteriology·R G EAGON
Dec 4, 2003·Biochemical and Biophysical Research Communications·Zigmund Luka, Conrad Wagner
Jan 24, 2004·Journal of Inherited Metabolic Disease·P Augoustides-SavvopoulouS H Mudd
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ivo BaricS Harvey Mudd
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Mar 25, 2006·Archives of Disease in Childhood. Fetal and Neonatal Edition·Catherine A BoydPeter Brocklehurst
Aug 3, 2006·Pediatrics·John PendersEllen E Stobberingh
Jan 27, 2007·Genome Research·Daniel H HusonStephan C Schuster
Apr 27, 2007·Journal of Pediatric Gastroenterology and Nutrition·Marie-José ButelChristophe Dupont
Jun 28, 2007·PLoS Biology·Chana PalmerPatrick O Brown
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Jan 16, 2009·Acta Paediatrica·I Adlerberth, A E Wold
Jun 6, 2009·The Journal of Physiology·Peter J Turnbaugh, Jeffrey I Gordon
Jul 16, 2009·Environmental Microbiology·Julien TapMarion Leclerc
Aug 12, 2009·Nature Methods·Christopher QuinceWilliam T Sloan
Aug 19, 2009·Journal of Microbiological Methods·Laurent RoudièreEstelle Jumas-Bilak
Nov 13, 2009·Nutrition Reviews·Lars Bode
Mar 20, 2010·Environmental Microbiology·Susan M HuseMitchell L Sogin

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Citations

Mar 19, 2021·Frontiers in Cellular and Infection Microbiology·Clara Yieh Lin ChongJustin M O'Sullivan
Aug 12, 2021·Computational and Structural Biotechnology Journal·Zhen YangYungang He
Aug 31, 2021·Frontiers in Cellular and Infection Microbiology·Jie ChenXiaomei Cong

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Datasets Mentioned

BETA
PRJNA515307

Methods Mentioned

BETA
amplicon sequencing
cesarean section
RNA-seq
PCR
16S sequencing
nucleic acid extraction

Software Mentioned

Join
GraphPad Prism
Fastq
Quantitative Insights Into Microbial Ecology 2 ( QIIME2
Python scripts
LEfSe
MEGAN6
USEARCH
ANCOM
BioShare

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