Comparing immunological and molecular tests with conventional methods in diagnosis of acute invasive fungal rhinosinusitis

Journal of Infection in Developing Countries
Parisa BadieeHossain Rozbehani

Abstract

This study compared immunological and molecular testswith conventional methods in the diagnosis of acute invasive fungal rhinosinusitis. All tissue samples from maxillary sinuses were cultured and stained using periodic acid-Schiff. Two blood samples were cultured by bedside inoculation into BACTEC medium. Diagnostic tests were used: enzyme-linked immunosorbent assay for detection of galactomannan and mannan in serum samples, real-time PCR assays for Aspergillus spp. and Candida spp., and nested PCR for the Mucoraceae family. Among 31 immunocompromised patients, based on host factors, clinical, radiological and mycological findings, 18 patients were diagnosed with documented infection. By direct smear examination, 9 patients (50%) had positive results for Mucoraceae family, but only 5 samples showed growth in the culture (55.5%). Nested PCR results for species in this family were negative in all serum samples from patients. In addition, 9 patients had positive direct microscopic findings, of which 7 specimens produced positive growth for Aspergillus flavus (77.7%). The galactomannan test was positive in 6/9 (66.6%), and Aspergillus PCR were positive in 6 patients (66.6%). The isolated agent was C. albicans in one patient. The m...Continue Reading

Citations

Sep 4, 2019·The Cochrane Database of Systematic Reviews·Mario CrucianiLewis P White
Apr 7, 2019·BMC Infectious Diseases·Mariana L C SilveiraFabiana C P Valera
May 8, 2021·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Suk Won ChangHyung-Ju Cho

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