Comparing inactivation protocols of Yersinia organisms for identification with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Carine CoudercMichel Drancourt

Abstract

It is recommended that harmful Biosafety Level 3 (BSL-3) bacteria be inactivated prior to identification by mass spectrometry, yet optimal effects of inactivation protocol have not been defined. Here, we compare trifluoroacetic acid inactivation (protocol A) with ethanol inactivation (protocol B) of Yersinia organisms prior to identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The total number of peaks detected was 10.5 ± 1.7 for protocol A and 15.7 ± 4.2 for protocol B (ρ <0.001, ANOVA test). The signal-to-noise ratio for the m/z 6049 peak present in all of the tested Yersinia isolates was 9.7 ± 3.1 for protocol A and 18.1 ± 4.6 for protocol B (ρ < 0.001). Compared with spectra in our local database containing 48 Yersinia spp., including 20 strains of Y. pestis, the identification score was 1.79 ± 0.2 for protocol A and 1.97 ± 0.19 for protocol B (ρ = 0.0024). Our observations indicate that for the identification of Yersinia organisms, ethanol inactivation yielded MALDI-TOF-MS spectra of significantly higher quality than spectra derived from trifluoroacetic acid inactivation. Combined with previously published data, our results permit the updating of protocols for ina...Continue Reading

References

Oct 3, 1999·Applied and Environmental Microbiology·Y HathoutC Fenselau
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Nov 13, 2007·Diagnostic Microbiology and Infectious Disease·Ellen Jo Baron, J Michael Miller
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Jul 1, 2011·The New England Journal of Medicine·Karen M FrankWun-Ju Shieh

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Citations

Dec 4, 2012·Journal of Microbiological Methods·Dobryan M TraczCindi R Corbett
Aug 25, 2015·Frontiers in Microbiology·Neelja SinghalJugsharan S Virdi
Jan 6, 2017·Scientific Reports·Maliya Alia MalekMichel Drancourt

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