Comparing strategies for deep astigmatism-based single-molecule localization microscopy

Biomedical Optics Express
Marijn SiemonsLukas C Kapitein

Abstract

Single-molecule localization microscopy (SMLM) enables fluorescent microscopy with nanometric resolution. While localizing molecules close to the coverslip is relatively straightforward using high numerical aperture (NA) oil immersion (OI) objectives, optical aberrations impede SMLM deeper in watery samples. Adaptive optics (AO) with a deformable mirror (DM) can be used to correct such aberrations and to induce precise levels of astigmatism to encode the z-position of molecules. Alternatively, the use of water immersion (WI) objectives might be sufficient to limit the most dominant aberrations. Here we compare SMLM at various depths using either WI or OI with or without AO. In addition, we compare the performance of a cylindrical lens and a DM for astigmatism-based z-encoding. We find that OI combined with adaptive optics improves localization precision beyond the performance of WI-based imaging and enables deep (>10 µm) 3D localization.

References

Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Martin J BoothTony Wilson
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Oct 4, 2007·Journal of Microscopy·M SchwertnerT Wilson
May 13, 2008·Nature Methods·Manuel F JuetteJoerg Bewersdorf
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sri Rama Prasanna PavaniW E Moerner
Dec 18, 2010·Optics Express·Sjoerd Stallinga, Bernd Rieger
May 9, 2012·Annual Review of Biomedical Engineering·Travis J GouldJoerg Bewersdorf
Oct 11, 2014·Physical Review Letters·Yoav ShechtmanW E Moerner
May 28, 2016·Biomedical Optics Express·Benjamin C ColesValentina Lo Schiavo
Nov 20, 2018·Nano Letters·Roderick P TasLukas C Kapitein

❮ Previous
Next ❯

Citations

Mar 25, 2020·Biomedical Optics Express·Peter KnerSjoerd Stallinga
Jun 9, 2021·Nature Communications·Marijn E SiemonsLukas C Kapitein
May 21, 2021·The Journal of Biological Chemistry·Jessica ValliColin Rickman
Jun 25, 2021·Quarterly Reviews of Biophysics·David J DeRosier
Jul 30, 2021·Nature Communications·Jan N HansenLuis Alvarez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.