Comparing the effect of IL-12 genetic adjuvant and alum non-genetic adjuvant on the efficiency of the cocktail DNA vaccine containing plasmids encoding SAG-1 and ROP-2 of Toxoplasma gondii.

Parasitology Research
Kamy Hosseinian KhosroshahiFariba Khoshzaban

Abstract

Various methods are available for enhancing the potency of DNA vaccines, including employment of different forms of adjuvant. The current study was carried out to evaluate and compare the effects of genetic and non-genetic adjuvants on the immune response stimulated by DNA vaccine. Thus, two adjuvants, IL-12 (genetic adjuvant) and aluminum hydroxide (alum, non-genetic adjuvant), were used with cocktail DNA vaccine containing plasmids encoding complete rhoptry antigen 2 (ROP-2) and surface major antigen 1 (SAG-1) of Toxoplasma gondii. The efficacy of pcROP2+pcSAG1 in stimulation of the immune response against toxoplasmosis with and without adjuvant was evaluated in female BALB/c mice by measuring the level of total IgG antibody and cytokines. The results obtained indicated that after challenging the mice with the fatal RH strain of T. gondii, the survival rates of mice immunized with pcROP2+pcSAG1 (DNA cocktail), pcSAG1+pcROP2+alum, and pcSAG1+pcROP2+IL-12 were significantly greater than that of the control groups (p<0.05). Moreover, measurement of total IgG antibody indicated the significant difference between the control and experimental groups (p<0.05). Finally, the results obtained by measurement of cytokines (IFN-γ and IL-4...Continue Reading

References

Jun 1, 1988·Molecular and Biochemical Parasitology·A SadakJ F Dubremetz
Nov 7, 1999·Molecular and Biochemical Parasitology·S HunterM Milhausen
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SinghD O'Hagan
Aug 28, 2001·Molecular and Biochemical Parasitology·C F ZineckerR T Schwarz
Aug 28, 2001·Veterinary Parasitology·W Kofta, H Wedrychowicz
May 10, 2003·Microbes and Infection·Girish M Bhopale
Sep 27, 2003·Methods : a Companion to Methods in Enzymology·Shin SasakiKenji Okuda
Aug 9, 2006·Clinical and Vaccine Immunology : CVI·Ken S Rosenthal, Daniel H Zimmerman
Aug 19, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·A Crampton, T Vanniasinkam
May 12, 2009·Memórias do Instituto Oswaldo Cruz·Erik JongertEskild Petersen

❮ Previous
Next ❯

Citations

Sep 8, 2012·Parasitology Research·Jong-Hyun KimHo-Joon Shin
Aug 21, 2013·Parasitology Research·Yanhua WangMeng Wang
Mar 13, 2013·Expert Opinion on Biological Therapy·Lorenzo Antonio RamirezJean Boyer
Dec 15, 2012·Vaccine·Benoît LevastVolker Gerdts
Sep 18, 2012·Journal of Biotechnology·Lei LiNikolai Petrovsky
Jul 8, 2015·AIDS Research and Human Retroviruses·Daniela Santoro RosaEdecio Cunha-Neto
Dec 7, 2014·Asian Pacific Journal of Tropical Medicine·Hajar Ziaei HezarjaribiZohreh Sharifi
Apr 2, 2013·Experimental Parasitology·Hajar Ziaee HezarjaribiOgholniaz Jorjani
Apr 11, 2018·Parasite Immunology·N Silva-GutierrezS Salmen
Dec 29, 2015·Expert Review of Vaccines·Lei Li, Nikolai Petrovsky
Feb 6, 2018·Clinical and Experimental Vaccine Research·Masoud Foroutan, Fatemeh Ghaffarifar
Oct 9, 2019·Folia Parasitologica·Junxin XueQuan Wang
Feb 19, 2019·Clinical and Experimental Vaccine Research·Masoud ForoutanOgholniaz Jorjani
Aug 17, 2018·Clinical and Experimental Vaccine Research·Masoud ForoutanFatemeh Ghaffarifar
Jun 25, 2015·Microbiology Spectrum·John S Tregoning, Ekaterina Kinnear

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.