Comparing the permeability of human and porcine small intestinal mucus for particle transport studies.

Scientific Reports
Lukasz KrupaAdam Macierzanka

Abstract

The gastrointestinal mucus layer represents the last barrier between ingested food or orally administered pharmaceuticals and the mucosal epithelium. This complex gel structure plays an important role in the process of small intestinal absorption. It provides protection against hazardous particles such as bacteria but allows the passage of nutrients and drug molecules towards the intestinal epithelium. In scientific research, mucus from animal sources is usually used to simulate difficult-to-obtain human small intestinal mucus for investigating the intramucus transport of drug delivery systems or food nanoparticles. However, there is a lack of evidence the human mucus can be reliably substituted by animal counterparts for human-relevant transport models. In this report, a procedure for collecting human mucus has been described. More importantly, the permeability characteristics of human and porcine small intestinal mucus secretions to sub-micron sized particles have been compared under simulated intestinal conditions. Negatively charged, 500 nm latex beads were used in multiple-particle tracking experiments to examine the heterogeneity and penetrability of mucus from different sources. Diffusion of the probe particles in adult ...Continue Reading

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Citations

May 31, 2021·Advanced Drug Delivery Reviews·Zhi LuoJean-Christophe Leroux
Jun 12, 2021·Food Research International·Dorota DulkoAdam Macierzanka
May 30, 2021·Advanced Healthcare Materials·Kevin PengSamir Mitragotri
Aug 20, 2021·Advanced Drug Delivery Reviews·Yuandong ZhangXun Sun

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Methods Mentioned

BETA
biopsy
Scanning electron microscopy
dynamic light scattering
confocal microscopy

Software Mentioned

Pro Analyzer
Image

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