Comparing the properties of Escherichia coli branching enzyme and maize branching enzyme

Archives of Biochemistry and Biophysics
H GuanP Keeling

Abstract

Escherichia coli glycogen branching enzyme (GBE) and maize starch branching enzymes I (SBEI) and II (SBEII) were expressed in E. coli and purified. E. coli GBE branched amylose at a higher rate than did SBEII, but branched amylose at a lower rate than did SBEI. Similar to SBEI, GBE branched amylopectin at a lower rate than did SBEII. High-performance anion-exchange chromatography analysis of the branched products produced by BE revealed the minimum chain length (cl) required for branching. While GBE and SBEII showed the same minimum cl [degree of polymerization (dp) 12] required for branching, SBEI had a slightly higher minimum cl (dp 16) requirement for branching. The major differences between GBE and SBE are their specificities in terms of the size of chains transferred. In comparison with SBE, GBE had a much narrower size range of chains transferred and transferred mainly shorter chains. While SBEI and SBEII produced a large number of chains ranging from dp 6 to over dp 30, GBE predominantly transferred chains ranging from dp 5 to 16 and produced only a very small number of long chains with dp greater than 20. Although it has been reported that SBEI and SBEII preferentially transfer longer and shorter chains, respectively (1...Continue Reading

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Citations

Apr 22, 2009·Plant & Cell Physiology·Takayuki SawadaYasunori Nakamura
Mar 23, 2010·Plant & Cell Physiology·Yasunori NakamuraShinichi Kitamura
Apr 22, 2011·Plant Physiology·Marna D Yandeau-NelsonMark J Guiltinan
Mar 23, 2011·Journal of Bacteriology·Jong-Tae ParkKwan-Hwa Park
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May 12, 2016·Cellular and Molecular Life Sciences : CMLS·Barbara Pfister, Samuel C Zeeman
May 26, 2016·Biochemistry·Abdul M RashidStephen Bornemann
Feb 7, 2017·Biochemical and Biophysical Research Communications·Soohui NaNam-Chul Ha
Jan 14, 2017·International Journal of Biological Macromolecules·Yiting LiuZhaofeng Li
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Dec 6, 2001·European Journal of Biochemistry·U RydbergH Larsson
Aug 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Mari HayashiEiji Suzuki
Apr 20, 2017·Frontiers in Plant Science·Juan WangCunxu Wei
Sep 28, 2017·Analytical and Bioanalytical Chemistry·Sharif S NadaRobert G Gilbert
Jan 31, 2020·Critical Reviews in Biotechnology·Xiaofeng BanZhaofeng Li
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May 4, 2016·Acta Crystallographica. Section D, Structural Biology·Lei FengJames H Geiger
Aug 2, 2019·The Biochemical Journal·Javier O CifuenteMarcelo E Guerin

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