Comparison analysis of distributions of tyrosine hydroxylase, calmodulin and calcium/calmodulin-dependent protein kinase II in a triple stained slice of rat brain

Brain Research
D SutooK Yabe

Abstract

The immunohistochemical distributions of tyrosine hydroxylase (TH), calmodulin (CaM) and calcium/CaM-dependent protein kinase II (CaMKII) in the rat forebrain were compared quantitatively to confirm our previous finding that TH activity and dopamine synthesis in the brain are regulated by a calcium/CaM-dependent system. The same slice was triply stained and the above substances were detected immunohistochemically. Their distributions in the slice were measured using a brain mapping analyzer which is a microphotometry system for the analysis of the distribution of neurochemicals in a large tissue slice. Each coronal section was divided into approximately 250000 to 310000 microareas at 20-microm intervals, and the immunohistochemical fluorescence intensities of the three substances in these microareas were analyzed independently. Quantitative images of the distributions were reconstructed from the data, and the distribution of each substance was investigated. Immunoreactive staining of TH, CaM and CaMKII was observed in almost all areas of the brain, but the intensities varied. Markedly intense TH-, CaM- and CaMKII-like immunoreactivities were distributed in the anterior dorsolateral and posterior areas of the neostriatum, nucleu...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·P I Hanson, H Schulman
Jul 1, 1990·Trends in Neurosciences·A M Graybiel
Jan 1, 1985·Annual Review of Biochemistry·A C NairnP Greengard
Nov 1, 1989·Trends in Neurosciences·M B Kennedy
Jul 1, 1985·Journal of Neurochemistry·M D BrowningP Greengard
Mar 1, 1989·Brain Research Bulletin·D SutooM Geffard
Jun 1, 1989·Japanese Journal of Pharmacology·D SutooK Matsushita
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A M GraybielY A Agid
May 1, 1985·Journal of Neurochemistry·L W ZhouB Weiss
Oct 1, 1985·Pharmacology, Biochemistry, and Behavior·D SutooK Iimura
Sep 1, 1971·Journal of Neurochemistry·E G McGeerJ A Wada
Jan 1, 1983·Histochemistry·A Seto-OhshimaA Mizutani
Jan 1, 1984·Experimental Brain Research·A BiberK Hempel
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C C OuimetP Greengard
Feb 27, 2001·Journal of Neuroscience Research·D SutooK Yabe

❮ Previous
Next ❯

Citations

Apr 9, 2014·Genome Biology and Evolution·Makoto NasuShintaroh Ueda
Feb 14, 2019·The Journal of Physiological Sciences : JPS·Kayo NakamuraJunichi Nabekura
Oct 8, 2014·Translational Psychiatry·A C EastonC P Müller
May 8, 2013·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasushi YabukiKohji Fukunaga
Dec 18, 2015·Trends in Pharmacological Sciences·Christian P MüllerK Peter Giese
May 22, 2003·Neurobiology of Disease·Den'etsu Sutoo, Kayo Akiyama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.