Comparison and evaluation of experimental mediastinitis models: precolonized foreign body implants and bacterial suspension inoculation seems promising.

BMC Infectious Diseases
Gulden ErsozMurat Dikmengil

Abstract

Post-sternotomy mediastinitis (PSM) is a devastating surgical complication affecting 1-3% of patients that undergo cardiac surgery. Staphylococcus aureus is one of the most commonly encountered bacterial pathogen cultured from mediastinal samples obtained from patients with PSM. A component of the membrane of the gram positive bacteria, lipoteichoic acid, stimulates the blood monocytes and macrophages to secrete cytokines, radicals and nitrogen species leading to oxido-inflammatory damage. This seems to be responsible for the high mortality rate in PSM. For the evaluation of the pathogenesis of infection or for the investigation of alternative treatment models in infection, no standard model of mediastinitis seems to be available. In this study, we evaluated four mediastinitis models in rats. The rats were divided into four groups to form different infection models. Group A: A suspension of 1 x 107 colony-forming units Staphylococcus aureus in 0,5 mL was inoculated from the right second intercostal space into the mediastinum. Group B: A hole was created in the right second intercostal space and a piece of stainless-steel implant with a length of 0.5 cm was inserted into the mediastinum and a suspension of 1 x 107 cfu bacteria i...Continue Reading

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Citations

Sep 19, 2008·The Journal of Antimicrobial Chemotherapy·Yoav BarneaShiri Navon-Venezia
Jun 16, 2009·Journal of the Neurological Sciences·Lubin FangJohannes Brettschneider
Feb 3, 2009·The Journal of Surgical Research·Vedat TurhanOral Oncul

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