Comparison and Recommendations for Use of Dientamoeba fragilis Real-Time PCR Assays

Journal of Clinical Microbiology
Rory GoughDamien Stark

Abstract

Dientamoeba fragilis is a gastrointestinal trichomonad parasite whose pathogenicity is yet to be determined. The difficulty involved in microscopically diagnosing D. fragilis in feces led to the development of real-time PCR methodologies for the detection of D. fragilis in stool samples. Prevalence studies in Europe show much higher levels of infection where a laboratory-developed real-time assay is the predominant assay for the detection of Dientamoeba fragilis than in regions that use the EasyScreen assay for detection of gastrointestinal pathogens. The aim of this study was to compare a commercially available Dientamoeba fragilis assay (Genetic Signatures EasyScreen assay) to a widely used laboratory-developed real-time PCR method. Two hundred fifty fecal samples were screened using the laboratory-developed real-time assay on four real-time PCR platforms producing a number of discrepant results. Limit-of-detection studies were undertaken to attempt to resolve sensitivity for each platform tested. The presence or absence of Dientamoeba fragilis DNA in discrepant samples was shown using PCR amplicon next-generation sequencing. Eukaryotic 18S diversity profiling was conducted on discrepant samples to identify the presence or ab...Continue Reading

References

Jan 1, 1977·The American Journal of Tropical Medicine and Hygiene·J Yang, T Scholten
Nov 1, 1989·The Pediatric Infectious Disease Journal·M Ish-HorowiczR J Deckelbaum
Sep 1, 1966·The American Journal of Digestive Diseases·B H Kean, C L Malloch
Apr 1, 1983·Digestive Diseases and Sciences·V MilletL S Garcia
Feb 18, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N GirginkardeşlerU Z Ok
Apr 15, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A NorbergB Evengård
Jul 17, 2003·The Journal of Emergency Medicine·Michael D Schwartz, Michael E Nelson
Dec 29, 2005·Trends in Parasitology·Damien J StarkJohn Harkness
Feb 14, 2006·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Olivier VandenbergTom van Gool
Jan 24, 2007·Journal of Travel Medicine·Damien StarkJohn Harkness
Jun 26, 2007·Molecular and Cellular Probes·Jaco J VerweijLisette van Lieshout
Mar 30, 2010·The American Journal of Tropical Medicine and Hygiene·Damien StarkJohn Ellis
May 14, 2010·Diagnostic Microbiology and Infectious Disease·Adriana CalderaroCarlo Chezzi
Nov 15, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christina M CoyleHerbert B Tanowitz
Feb 4, 2012·The American Journal of Tropical Medicine and Hygiene·Damien StarkJohn T Ellis
Nov 3, 2012·Experimental Parasitology·Dennis RöserChristen Rune Stensvold
Apr 24, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D RöserK Mølbak
Jun 3, 2014·BMC Infectious Diseases·Adriana CalderaroMaria Cristina Arcangeletti
Jul 9, 2014·Annual Review of Microbiology·Chelsea Marie, William A Petri
Aug 14, 2015·PLoS Pathogens·Julius LukešLaura Wegener Parfrey
May 14, 2016·Clinical Microbiology Reviews·Damien StarkJohn T Ellis
Aug 16, 2016·Veterinary Parasitology·Douglas ChanDamien Stark
Mar 24, 2017·Journal of Clinical Microbiology·Pikka JokelainenChristen R Stensvold

❮ Previous
Next ❯

Citations

Jul 25, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Jukka-Pekka PietiläAnu Kantele
Apr 14, 2020·International Journal of Microbiology·Muhammad Amjad
Nov 15, 2020·Journal of Microbiological Methods·Luis Enrique Jerez PueblaLucy J Robertson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved