Comparison between conventional culture and NAATs for the microbiological diagnosis in gonococcal infection

Diagnostic Microbiology and Infectious Disease
J Serra-PladevallA Andreu

Abstract

Gonorrhea is a public health problem. Fast diagnostics is necessary. The aim of this study was to compare the diagnostic yield of culture with that of polymerase chain reaction (PCR) in gonococcal infection. This is a study comparing the results of Neisseria gonorrhoeae detection by culture versus PCR from July to December 2012. Molecular diagnosis was performed by real-time PCR using the Versant CT/GC DNA 1.0 assay. In the 768 specimens, 96.9% the results were concordant. In 3.1%, the results were discordant, being PCR-positive and culture-negative in 21 cases and PCR-negative and culture-positive in 3. The sensitivity, specificity, positive predictive value, and negative predictive value for culture were 86.2%, 99.8%, 99.2%, and 96.7%, and for PCR, 98.7%, 100%, 100% and 99.7%, respectively. In laboratories where antimicrobial susceptibility is monitored, an effective approach would be to perform culture in addition to PCR in symptomatic patients.

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Citations

Aug 12, 2016·International Journal of STD & AIDS·Rechel GeigerSanjay R Mehta
Mar 29, 2019·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Susanne BuderNorbert Brockmeyer
Mar 29, 2019·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Susanne BuderNorbert Brockmeyer
Mar 12, 2016·Sexually Transmitted Diseases·Vivian LevyIna U Park
Oct 31, 2018·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·Susanne Buder
Aug 27, 2021·Sexually Transmitted Diseases·Evelyn E NashUNKNOWN SURRG Working Group

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