Comparison between Escherichia coli K-12 strains W3110 and MG1655 and wild-type E. coli B as platforms for xylitol production

Biotechnology Letters
Reza KhankalPatrick C Cirino

Abstract

Escherichia coli W3110 was previously engineered to produce xylitol from a mixture of glucose plus xylose by expressing xylose reductase (CbXR) and deleting xylulokinase (DeltaxylB), combined with either plasmid-based expression of a xylose transporter (XylE or XylFGH) (Khankal et al., J Biotechnol, 2008) or replacing the native crp gene with a mutant (crp*) that alleviates glucose repression of xylose transport (Cirino et al., Biotechnol Bioeng 95:1167-1176, 2006). In this study, E. coli K-12 strains W3110 and MG1655 and wild-type E. coli B were compared as platforms for xylitol production from glucose-xylose mixtures using these same strategies. The engineered strains were compared in fed-batch fermentations and as non-growing resting cells. Expression of CRP* in the E. coli B strains tested was unable to enhance xylose uptake in the presence of glucose. Xylitol production was similar for the (crp*, DeltaxylB)-derivatives of W3110 and MG1655 expressing CbXR (average specific productivities of 0.43 g xylitol g cdw(-1 ) h(-1) in fed-batch fermentation). In contrast, results varied substantially between different DeltaxylB-derivative strains co-expressing either XylE or XylFGH. The differences in genetic background between these...Continue Reading

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Mar 25, 2008·Journal of Biotechnology·Reza KhankalPatrick C Cirino

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Citations

Dec 17, 2009·Applied Microbiology and Biotechnology·Miho SasakiHideaki Yukawa
Jul 16, 2013·Journal of Agricultural and Food Chemistry·Chung-Jen ChiangYun-Peng Chao
Aug 26, 2009·Journal of Biological Engineering·Reza KhankalPatrick C Cirino
Nov 26, 2015·Nanotoxicology·Alexandre GelabertRoselyne Ferrari
Sep 2, 2008·Current Opinion in Biotechnology·Olubolaji AkinterinwaPatrick Carmen Cirino
Jan 17, 2012·Chemosphere·Marcos PileggiMichael Jay Sadowsky
Feb 6, 2020·Biotechnology for Biofuels·Zhe ZhaoGuang Zhao
Feb 11, 2021·Critical Reviews in Biotechnology·Angelo B BañaresWook-Jin Chung
Oct 28, 2017·Journal of Agricultural and Food Chemistry·Mukesh SainiYun-Peng Chao

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