PMID: 9027440Dec 1, 1996

Comparison between hypertonic and isotonic saline-induced sputum in the evaluation of airway inflammation in subjects with moderate asthma

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Elena BacciC Giuntini

Abstract

Hypertonic saline-induced sputum has recently been used for the evaluation of airway inflammation in asthma. To assess the effect of hypertonicity on airway inflammation. We compared the inflammatory cell composition of hypertonic saline-induced sputum with that of isotonic saline-induced sputum in 21 asthmatic subjects and, at baseline and 30 min after each sputum induction, we measured bronchial hyper-responsiveness to methacholine as an indirect marker to detect increased airway inflammation. On two different days, the patients inhaled hypertonic saline (3-5% NaCl) or isotonic saline (0.9% NaCl) for 30 min via an ultrasonic nebulizer, while monitoring FEV1. Sputum was collected for inflammatory cell analysis. There was no difference in inflammatory cell percentages obtained with the two methods. Eosinophils were > 1% in 20 subjects after hypertonic saline and in 16 subjects after isotonic saline, but this difference was not statistically significant. Intraclass correlation coefficients for sputum inflammatory cells obtained with the two methods were +0.642 for eosinophils, +0.644 for neutrophils, +0.544 for lymphocytes and +0.505 for macrophages. Hypertonic saline induced bronchoconstruction in a significantly greater number...Continue Reading

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Related Concepts

Eosinophil
Bronchial System
Normal saline
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Neutrophils as Percentage of Blood Leukocytes (Lab Test)
Muscle Hypertonia
Bronchial Provocation Tests
Sputum, Induced
Differential White Blood Cell Count Procedure
Bronchoconstriction

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