Comparison between In Vitro Antiviral Effect of Mexican Propolis and Three Commercial Flavonoids against Canine Distemper Virus

Evidence-based Complementary and Alternative Medicine : ECAM
María de Jesús González-BúrquezSalvador Fonseca-Coronado

Abstract

Propolis is a resin that honey bees (Apis mellifera) produce by mixing wax, exudates collected from tree shoots, pollen, and enzymes. It has been used for its biological properties against pathogenic microorganisms including those of viral origin. In the present study, we demonstrate the antiviral effect of Mexican propolis, as well as of the three commercial flavonoids (quercetin, naringenin, and pinocembrin) present in its composition, in cell cultures infected with Canine Distemper Virus. The treatments were carried out with propolis, flavonoids individually, and a mixture of the three flavonoids at three different times. Antiviral activity was evaluated by the inhibition of the relative expression of the virus nucleoprotein gene (Real-Time qPCR) and by the determination of cellular viability (MTT assay). Propolis applied before infection decreased viral expression (0.72 versus 1.0, 1.65, and 1.75 relative expressions) and correlated with increased cell viability (0.314 versus 0.215, 0.259, and 0.237 absorbance units (AU)). The administration of a flavonoid mixture containing the three commercial flavonoids before infection induces a slight decrease in viral expression (0.93 versus 1, 1.42, and 1.82 relative expressions); ho...Continue Reading

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Citations

Jun 9, 2020·Dermatologic Therapy·Dimitri BachevskiMaja Dimova
Aug 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magdalena Broda
Dec 31, 2020·Biomolecules·Vanessa Loaiza-CanoDamião Pergentino de Sousa
Jan 19, 2021·F1000Research·Bruno Silva AndradeVasco Ariston de Carvalho Azevedo
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Nicolas RipariJosé Maurício Sforcin
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Aug 28, 2021·Nutrients·Priyanshu BhargavaSunil C Kaul

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Datasets Mentioned

BETA
EF418783.1

Methods Mentioned

BETA
PCR

Software Mentioned

Primer3
GraphPad Prism

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