Comparison between quantitative real-time reverse transcription PCR results for norovirus in oysters and self-reported gastroenteric illness in restaurant customers

Journal of Food Protection
James A LowtherD N Lees

Abstract

Norovirus is the principal agent of bivalve shellfish-associated gastroenteric illness worldwide. Numerous studies using PCR have demonstrated norovirus contamination in a significant proportion of both oyster and other bivalve shellfish production areas and ready-to-eat products. By comparison, the number of epidemiologically confirmed shellfish-associated outbreaks is relatively low. This study attempts to compare norovirus RNA detection in Pacific oysters (Crassostrea gigas) by quantitative real-time reverse transcription PCR (RT-PCR) and human health risk. Self-reported customer complaints of illness in a restaurant setting (screened for credible norovirus symptoms) were compared with presence and levels of norovirus as determined by real-time RT-PCR for the batch of oysters consumed. No illness was reported for batches consistently negative for norovirus by real-time RT-PCR. However, norovirus was detected in some batches for which no illness was reported. Overall presence or absence of norovirus showed a significant association with illness complaints. In addition, the batch with the highest norovirus RNA levels also resulted in the highest rate of reported illness, suggesting a linkage between virus RNA levels and health...Continue Reading

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Citations

Nov 19, 2011·Journal of Applied Microbiology·R HartnellJ Russell
Feb 1, 2012·Journal of Food Protection·James A LowtherDavid N Lees
Jun 9, 2017·Foodborne Pathogens and Disease·Saiki ImamuraKeiko Akimoto
Jun 25, 2019·Transboundary and Emerging Diseases·Ravo M RazafimahefaEtienne Thiry
Oct 29, 2010·Journal of Public Health·Kenneth BakerMichael Young
May 4, 2018·Food and Environmental Virology·J A LowtherD N Lees
Oct 30, 2010·Emerging Infectious Diseases·Kirsten MattisonJeffrey M Farber
May 23, 2019·Food and Environmental Virology·J A LowtherD N Lees
Jul 7, 2020·EFSA Journal·UNKNOWN European Food Safety Authority (EFSA)
Jul 14, 2011·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)
Feb 4, 2014·Applied and Environmental Microbiology·Mu YeHaiqiang Chen
Jul 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Catherine McLeodFrançoise S Le Guyader
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Siele CeuppensJohn Donaghy
Jul 1, 2015·Environmental Science & Technology·Carlos J A CamposDavid N Lees

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