PMID: 7089989Mar 1, 1982Paper

Comparison between sensitivity of a viscometric method and sensitivity of the alkaline elution assay for the determination of DNA damage induced by dimethylsulfate in vitro

Toxicology Letters
S ParodiL Santi

Abstract

DNA damage induced by dimethylsulfate (DMS) was measured with a new oscillating crucible viscometer, having a U-shaped circular channel. Rat liver nuclei were treated in vitro. Viscosity was measured by lysing nuclei in an aklaline lysing solution (pH 12.5; 25 degrees C). Nuclei were lysed immediately in the viscometer and released DNA started to uncoil. In control samples the viscosity increased very slowly with time, reaching a maximum only after about 8 h. A progressively more rapid increase in viscosity was seen with increasing concentrations of DMS. The time of DNA disentanglement was sensitive to about 30 times less breaks than the alkaline elution assay.

Citations

Related Concepts

Dimethyl sulfate, 3H-labeled
Metazoa
Cell Nucleus
DNA, Double-Stranded
Hydrogen-Ion Concentration
Liver
Thiosulfuric Acid Esters
Sulfuric Acids
Viscosity
Rats, Laboratory

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