Comparison between the loss of platelet membrane asymmetry, microvesiculation and the tyrosine phosphorylation of proteins

Prostaglandins, Leukotrienes, and Essential Fatty Acids
J M PasquetA T Nurden

Abstract

Platelet activation by agents such as the Ca2+-ionophore A23187 or Ca2+-ATPase inhibitors leads to the generation of a procoagulant surface and the formation of microparticles. These responses are late events of platelet activation and readily detected by flow cytometry using annexin V-FITC as an aminophospholipid probe. One Ca2+-ATPase inhibitor, 2,5-di-(tertbutyl)-1,4-benzohydroquinone induced aminophospholipid exposure without microparticle formation. Previous work has shown that microparticle formation is strictly linked to the activation of calpain, a thiol-protease that modifies the platelet cytoskeleton and some signal transduction enzymes. We now report how the detection of platelet tyrosine phosphorylation by western-blotting clearly shows that when platelet activation and aminophospholipid exposure are accompanied by microparticle formation there is a decrease in the tyrosine phosphorylation of proteins.

References

Jul 16, 1990·Biochemical and Biophysical Research Communications·T InazuH Yamamura
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Golden, J S Brugge
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Ferrell, G S Martin
Jul 15, 1993·Biochemical and Biophysical Research Communications·H TakayamaM Okuma
Aug 1, 1996·European Journal of Biochemistry·J M PasquetA T Nurden
Dec 1, 1995·Nature Structural Biology·D BarfordN K Tonks

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