Comparison between the phenotype and function of maturing dendritic cells from spleen and lymph nodes

Immunology
J P CoatesS C Knight

Abstract

We compared the capacity of mature dendritic cells (DC) from lymph nodes and maturing DC from spleens in their capacity to stimulate responses to the small hapten picryl sulphonic acid (PIC) and to the same hapten conjugated to ovalbumin (PIC-OVA) and requiring processing. Surface expression of major histocompatibility complex (MHC) class II molecules, which are upregulated during maturation of splenic DC, were studied as an independent marker of maturation. Freshly isolated lymph node DC had a veiled appearance and high levels of class II expression. DC separated from suspensions of spleen cells expressed the DC-specific marker NLDC-145, but were small, had low levels of MHC class II molecules and expressed stem cell antigen. Those DC from spleen cells cultured for 24 and 48 hr showed the development of typical veiled DC morphology and high class II expression. Lymph node DC stimulated high levels of primary T-cell proliferation to PIC, but failed to stimulate primary responses to PIC-OVA. Splenic DC isolated immediately failed to stimulate primary responses to either antigen. More mature spleen DC stimulated responses both to PIC and PIC-OVA. Surprisingly, development of the capacity to stimulate responses to PIC preceded tha...Continue Reading

References

Apr 1, 1989·The Journal of Experimental Medicine·S E MacatoniaB A Askonas
Mar 1, 1985·The Journal of Experimental Medicine·G Schuler, R M Steinman
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S C KnightJ Clark
Dec 1, 1994·European Journal of Immunology·M MohamadzadehG Gradehandt

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Citations

May 19, 2000·Breast Cancer Research and Treatment·T TsugeM Tsukamoto
Jul 10, 2003·Immunology and Cell Biology·Heather L Wilson, Helen C O'Neill
Aug 12, 2006·American Journal of Respiratory Cell and Molecular Biology·Soren HansenJo Rae Wright

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