Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology

Theriogenology
Marcelo Emílio Beletti, Maria Luiza Silveira Mello

Abstract

Sperm chromatin alteration is an important feature that can affect fertility of the male rabbit. This study compared toluidine blue staining with Feulgen reaction (as methods for evaluating chromatin alteration) and investigated the relationship between sperm morphology and chromatin alteration. Seven hundred rabbit ejaculates of animals with unknown fertility were used. Primary and secondary morphological sperm abnormalities were evaluated in semen smears with phase-contrast microscopy. Chromatin alterations were evaluated in semen smears stained with toluidine blue (pH 4.0 and 5.0) and with the Feulgen reaction. While the three methods were equally efficacious for identification of chromatin alterations, toluidine blue staining was more appropriate to characterize the intensity of chromatin alterations. The correlation between primary sperm defects and chromatin alteration was high and positive, suggesting that sperm chromatin structure affected sperm head morphology. The correlation between secondary sperm defects and chromatin alteration was also positive, but lower. The final chromatin compaction occurs in the epididymus, where secondary sperm defects originate. Therefore, the causes of secondary sperm defects could also i...Continue Reading

Citations

Dec 24, 2005·Asian Journal of Andrology·J ErenpreissA Giwercman
Mar 6, 2008·Cell and Tissue Banking·Miguel Gustavo Setúbal AndradeMoysés Sadigursky
Jan 25, 2011·Urology·Alessandro Natali, Paul J Turek
May 9, 2008·Fertility and Sterility·Reda Z MahfouzAshok Agarwal
Nov 2, 2011·Andrologia·D Abu Hassan AbuR Henkel
Jul 15, 2016·Mechanisms of Ageing and Development·Igor PanticSenka Pantic
Feb 13, 2015·Biology of Reproduction·Sule DoganErdoğan Memili
Mar 7, 2018·Environmental Science and Pollution Research International·Ammar OumaimaMehdi Meriem
Oct 9, 2018·Microscopy Research and Technique·Luana Grasiele Pereira BezerraAlexandre Rodrigues Silva
Jun 3, 2009·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·G van der Horst, L Maree
Oct 12, 2019·Zygote : the Biology of Gametes and Early Embryos·Thais Rose Dos Santos Hamilton, Mayra Elena Ortiz D'Ávila Assumpção
May 8, 2018·International Journal of Biometeorology·Narian RomanelloAlexandre Rossetto Garcia
Jun 13, 2019·Reproduction in Domestic Animals = Zuchthygiene·Norma E MonachesiMaria Carretero
Aug 25, 2018·Histochemistry and Cell Biology·Jovana PaunovicIgor Pantic
Nov 1, 2017·Reproduction in Domestic Animals = Zuchthygiene·B R RuiRjg Pereira
Nov 26, 2008·The Veterinary Record·A L VieytesM R Ferrari
May 28, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jovana PaunovicIgor Pantic
Oct 8, 2018·Theriogenology·Ana Beatriz Bossois MouraAlexandre Rossetto Garcia

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.