Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR

Journal of Clinical Microbiology
Sophie CassaingJ F Magnaval

Abstract

PCR is now commonly applied to the diagnosis of toxoplasmosis. Although several methods are available, comparative studies are few, making it difficult to compare the performance of each technique. We compared the sensitivities of two real-time PCR assays through a prospective study on fetuses, neonates, and immunocompromised patients and on the ocular diagnosis of toxoplasmosis. The first system targeted the widely used B1 gene (GenBank accession number AF179871) while the second (RE) targeted a more recently described sequence repeated roughly 200 to 300 times (GenBank accession number AF146527). We demonstrated that molecular diagnosis requires the duplication of PCR assays, especially with the B1 system, as only one PCR was positive in 33.3% of cases. Our study showed that the RE target was more sensitive for all biological samples (amniotic fluid, placenta, aqueous humor, whole blood, and cerebrospinal and bronchoalveolar fluids) and significantly improved the performance of the diagnosis of toxoplasmosis. Taking into consideration all clinical samples, the mean gain in the crossing point value was 4.2 +/- 1.7 cycles and was even more significant for amniotic fluid (5.8 +/- 1.7 cycles).

References

Sep 15, 1994·The New England Journal of Medicine·P HohlfeldM Vidaud
Nov 4, 2000·Journal of Clinical Microbiology·M H LinC P Tseng
Oct 11, 2001·Bone Marrow Transplantation·J M CostaUNKNOWN Infectious Diseases Working Party of the European Group for Blood and Marrow Transplantation
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Patrick Bastien
May 6, 2003·Diagnostic Microbiology and Infectious Disease·Susanne BuchbinderArne Christian Rodloff
Dec 10, 2003·Journal of Medical Microbiology·Mehran J Marouni, Shlomo Sela
Apr 9, 2004·Journal of Clinical Microbiology·Elisabeth ChabbertPatrick Bastien
Dec 23, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rodrigo MartinoUNKNOWN Infectious Disease Working Party of the European Group for Blood and Marrow Transplantation

❮ Previous
Next ❯

Citations

May 29, 2007·Parasitology Research·Aurélien Dumètre, Marie-Laure Dardé
Sep 20, 2012·Environmental Science and Pollution Research International·Panagiotis KaranisKhalil M Khalil
Aug 28, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Carolina C CorreiaVláudia M A Costa
Aug 3, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Carolina C CorreiaVláudia M A Costa
Oct 28, 2009·The Pediatric Infectious Disease Journal·Florence Robert-GangneuxJean-Pierre Gangneux
Jan 24, 2008·Antimicrobial Agents and Chemotherapy·Pascale MeneceurFrancis Derouin
Apr 14, 2009·Applied and Environmental Microbiology·Wenli YangLihua Xiao
Dec 10, 2009·Future Microbiology·Vera Lucia Pereira-ChioccolaChunlei Su
Dec 3, 2008·Médecine et maladies infectieuses·D JametG Nevez
Apr 30, 2016·Jundishapur Journal of Microbiology·Mohammad MousaviSina Sekandarpour
Nov 17, 2015·Animal Health Research Reviews·Heidi M Wyrosdick, John J Schaefer
Dec 23, 2015·Parasite : Journal De La Société Française De Parasitologie·Nour MammariBertrand Courtioux
Feb 13, 2010·Journal of Medical Microbiology·Rafael T MesquitaVera L Pereira-Chioccola
Jun 13, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J MenottiF Derouin
Jan 27, 2018·Transactions of the Royal Society of Tropical Medicine and Hygiene·Japheth A OpintanMargaret Lartey
Feb 17, 2017·Journal of Clinical Microbiology·Florence Robert-GangneuxUNKNOWN Molecular Biology Study Group of the French National Reference Center for Toxoplasmosis
Nov 21, 2018·The American Journal of Tropical Medicine and Hygiene·Renzo Gutierrez-Loli For The Toxoplasmosis Working Group In Peru And Bolivia
Jan 14, 2019·The British Journal of Ophthalmology·Carlos A GomezThuy Doan
Mar 24, 2010·Obstetrics and Gynecology·Martine WallonFrançois Kieffer
Jan 30, 2020·Clinics·Helen Nazareth Veloso Dos SantosJoyce Hisae Yamamoto
May 19, 2016·Current Opinion in Infectious Diseases·Florence Robert-Gangneux, Sorya Belaz
Oct 18, 2016·Current Opinion in Ophthalmology·Thuy Doan, Benjamin A Pinsky
Jun 21, 2012·Clinical & Experimental Ophthalmology·Nicholas J ButlerJustine R Smith
May 25, 2015·Asian Pacific Journal of Tropical Medicine·Shirzad FallahiAli Haghighi
May 17, 2017·Parasitology Research·Deise F CostaAlessandra G Commodaro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Benjamin EdvinssonESCMID Study Group for Toxoplasmosis
Clinica Chimica Acta; International Journal of Clinical Chemistry
Bálint NagyZoltán Papp
Diagnostic Microbiology and Infectious Disease
Susanne BuchbinderArne Christian Rodloff
© 2021 Meta ULC. All rights reserved