Comparison in gene expression of secretory human endometrium using laser microdissection

Reproductive Biology and Endocrinology : RB&E
Atsushi YanaiharaTakashi Okai

Abstract

The endometrium prepares for implantation under the control of steroid hormones. It has been suggested that there are complicated interactions between the epithelium and stroma in the endometrium during menstrual cycle. In this study, we demonstrate a difference in gene expression between the epithelial and stromal areas of the secretory human endometrium using microdissection and macroarray technique. The epithelial and stromal areas were microdissected from the human endometrium during the secretory phase. RNA was extracted and amplified by PCR. Macroarray analysis of nearly 1000 human genes was carried out in this study. Some genes identified by macroarray analysis were verified using real-time PCR. In this study, changes in expression <2.5-fold in three samples were excluded. A total of 28 genes displayed changes in expression from array data. Fifteen genes were strongly expressed in the epithelial areas, while 13 genes were strongly expressed in the stromal areas. The strongly expressed genes in the epithelial areas with a changes >5-fold were WAP four-disulfide core domain 2 (44.1 fold), matrix metalloproteinase 7 (40.1 fold), homeo box B5 (19.8 fold), msh homeo box homolog (18.8 fold), homeo box B7 (12.7 fold) and protei...Continue Reading

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Citations

Aug 17, 2011·Reviews in Endocrine & Metabolic Disorders·Prajna Banerjee, Asgerally T Fazleabas
Oct 16, 2007·Gynecologic and Obstetric Investigation·Claire Bourgain, Paul Devroey
Dec 31, 2009·Reproductive Biology and Endocrinology : RB&E·Michal AmirEliezer Shalev
Mar 19, 2014·American Journal of Reproductive Immunology : AJRI·Carolina von GrothusenParameswaran G Lalitkumar
Dec 4, 2016·Genomics·Yueying WangMinggang Lei
Feb 24, 2006·Molecular Reproduction and Development·Jordi EstelléJosep M Folch
Nov 19, 2019·Molecular Reproduction and Development·Ruchi Kakar-BhanotGeetanjali Sachdeva
May 16, 2020·International Journal of Reproductive Medicine·Alison MacleanDharani K Hapangama
Aug 25, 2019·Medicina·Katiane de Almeida da CostaCarla de Azevedo Piccinato
Jun 6, 2007·Human Reproduction Update·K DiedrichUNKNOWN Evian Annual Reproduction (EVAR) Workshop Group
May 27, 2021·Clinical Epigenetics·Alejandra Monserrat Retis-ResendizEdgar Ricardo Vázquez-Martínez

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