Comparison of 2 culture methods and PCR assays for Salmonella detection in poultry feces

Poultry Science
M C SoriaDante J Bueno

Abstract

The present work compared 2 culture methods and the combinations of pre-enrichment and enrichment culture methods with PCR assays [buffered peptone water-PCR and tetrathionate-PCR or modified semisolid Rappaport-Vassiliadis (MSRV)-PCR] for motile and nonmotile Salmonella strain detection using artificially contaminated poultry feces. The specificity and positive predictive values were equal to one in both culture methods. Specificity and positive predictive values, accuracy, sensitivity, and negative predictive values were higher for motile than nonmotile Salmonella strains in culture methods. Only Salmonella enterica serovar Gallinarum was detected by the MSRV method with low accuracy, sensitivity, and negative predictive value. The detection level of motile strains was 2 ×10(0) to 22 × 10(2) cfu per 25 g for these methods, whereas it was 6.9 × 10(2) cfu per 25 g in culture methods for Salmonella Gallinarum. Extending the incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. In general, all selective plating media did not show any statistical differences in the parameters of performance studied. On the other hand, accuracy and sensitivity values were higher in MSRV-PCR and tetrat...Continue Reading

References

Jun 1, 1995·International Journal of Food Microbiology·M Busse
Feb 13, 2001·Letters in Applied Microbiology·N VoogtA W van de Giessen
Sep 13, 2001·Environmental Microbiology·J Guard-Petter
Sep 9, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Brenda C Love, Marcos H Rostagno
Feb 27, 2009·Zoonoses and Public Health·S Van HoorebekeJ Dewulf

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Citations

Oct 27, 2015·The Journal of Veterinary Medical Science·Masatoshi FujiharaKaho Uegaki
May 25, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Diego Felipe Alves BatistaAngelo Berchieri Junior
Aug 21, 2020·BMC Veterinary Research·Xin WangTengfei Zhang

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