Comparison of 2 high-throughput spectral techniques to predict differences in diet composition of grazing sheep and cattle.

Journal of Animal Science
J M MoorbyJ P Wold

Abstract

Diet composition can be estimated in free-ranging animals by the use of n-alkane and long-chain fatty alcohol concentrations in feces. However, this technique involves relatively laborious and costly analytical techniques. Two spectroscopy techniques were investigated as a way of determining whether dietary differences are likely, thus indicating whether the more expensive and labor-intensive techniques for more detailed analysis are justified. Fourier-transform infrared spectroscopy (FTIR) and front-face fluorescence emission spectroscopy (lambda(excitation) = 380 nm, lambda(emission) = 600 to 760 nm) were used to analyze fecal samples collected from 2 different breeds of cattle and sheep (4 groups in total, n = 6 per group) grazing moorland plants in 2 grazing sessions. These fecal samples were also analyzed for alkane and alcohol concentrations. Fourier-transform infrared spectra, particularly in the alkane regions, demonstrated clear separation between animal species. Fluorescence emission spectra showed similar separation; fluorophores were most likely chlorophylls and their derivatives. Multivariate analysis of all 3 data sets showed similar variation within and between groups of cattle and sheep, indicating differences i...Continue Reading

References

May 15, 2003·Journal of Agricultural and Food Chemistry·Kyle D AshbyJacob W Petrich
Jul 23, 2003·Journal of Food Protection·Moon S KimYud-Ren Chen
Nov 10, 2005·The British Journal of Nutrition·Alvaro BelenguerEric Milne
Nov 18, 2005·Applied Microbiology and Biotechnology·A BoschO Yantorno
Oct 31, 2006·Ecological Applications : a Publication of the Ecological Society of America·M D FraserJ M Moorby

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