Comparison of a biphasic non-radiometric system with Lowenstein-Jensen and Bactec-460 system for recovery of mycobacteria from clinical specimens

Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
M LuquinV Ausina

Abstract

The resurgence of tuberculosis and the increase of Mycobacterium avium complex infections have renewed interest in developing more efficient systems for isolation of mycobacteria. The Bactec radiometric method enhances the recovery and shortens the detection time of mycobacteria; however, many clinical laboratories are subject to severe restrictions on the utilization of 14C radiolabelled culture media. To compare the biphasic MB-Check system in the recovery of mycobacteria from clinical specimens with the Bactec-460 system and Lowenstein-Jensen (LJ) medium. The MB-Check was evaluated for routine use in a clinical laboratory by comparing the rates and times for isolation of mycobacteria from 1840 clinical specimens with results obtained using the other systems. The isolated organisms were identified by standard procedures and specific deoxyribonucleic acid (DNA) probes. The rate of recovery of all mycobacteria with the MB-Check system was 84.8%, compared to 87.5% for the Bactec-460 system and only 64.2% on LJ. Of the 147 M. tuberculosis isolates recovered by all methods combined, 92.5% were recovered by MB-Check, 87.1% by Bactec and 79.6% on LJ. Of the 109 isolates of nontuberculous mycobacteria (NTM) recovered by all methods c...Continue Reading

References

Jun 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T Giger, H J Burkardt

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