Comparison of a Chemically Mediated and an Immunologically Mediated Demyelinating Lesion Model

Methods : a Companion to Methods in Enzymology
R ReynoldsS J Piddlesden

Abstract

The production of two animal models for the central nervous system degenerative condition multiple sclerosis is described in detail. The first is a chemically mediated noninflammatory demyelinating lesion of the brain stem induced by the injection of a trypanocidal DNA binding dye, ethidium bromide, into the cerebellomedullary cistern. The injection does not involve any physical damage to the blood-brain barrier or the CSF-brain barrier and is simple to perform. The second lesion model is an immunologically mediated demyelinating condition involving the injection of a T-cell line specific for myelin basic protein, followed by injection of a monoclonal antibody against the myelin surface protein, myelin/oligodendrocyte glycoprotein. We describe the production of the antigen-specific T-cell line in detail. This model is characterized by widespread inflammatory infiltrates accompanied by areas of demyelination. Both of these models are produced in the Lewis rat, allowing the direct comparison of mechanisms involved in demyelination and repair in the presence or absence of invading inflammatory cells. Despite the very different etiologies of the two lesion models, they are both acute and result in efficient remyelination.

Citations

Aug 23, 2008·Biomechanics and Modeling in Mechanobiology·David I ShreiberRagi A I Elias
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·S PalmerJ Coffin
Jan 1, 2012·Multiple Sclerosis and Related Disorders·Markus KippSandra Amor
Jun 24, 2004·Journal of Neuroscience Research·Luc Jasmin, Peter T Ohara
Aug 9, 2008·Microvascular Research·Luciano A Guerreiro-LucasChristopher A Mitchell

❮ Previous
Next ❯

Related Feeds

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.