Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models

Journal of Biomedical Optics
Ali MaawyMichael Bouvet

Abstract

The aim of this study was to evaluate a set of visible and near-infrared dyes conjugated to a tumor-specific chimeric antibody for high-resolution tumor imaging in orthotopic models of pancreatic cancer. BxPC-3 human pancreatic cancer was orthotopically implanted into pancreata of nude mice. Mice received a single intravenous injection of a chimeric anti-carcinoembryonic antigen antibody conjugated to one of the following fluorophores: 488-nm group (Alexa Fluor 488 or DyLight 488); 550-nm group (Alexa Fluor 555 or DyLight 550); 650-nm group (Alexa Fluor 660 or DyLight 650), or the 750-nm group (Alexa Fluor 750 or DyLight 755). After 24 h, the Olympus OV100 small-animal imaging system was used for noninvasive and intravital fluorescence imaging of mice. Dyes were compared with respect to depth of imaging, resolution, tumor-to-background ratio (TBR), photobleaching, and hemoglobin quenching. The longer wavelength dyes had increased depth of penetration and ability to detect the smallest tumor deposits and provided the highest TBRs, resistance to hemoglobin quenching, and specificity. The shorter wavelength dyes were more photostable. This study showed unique advantages of each dye for specific cancer imaging in a clinically relev...Continue Reading

Citations

Nov 17, 2015·Expert Review of Anticancer Therapy·Jonathan DeLongMichael Bouvet
May 3, 2015·Molecular Immunology·Amanda C Freise, Anna M Wu
Mar 24, 2015·The Journal of Surgical Research·Ali A MaawyMichael Bouvet
Mar 25, 2015·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Yukihiko HiroshimaMichael Bouvet
Feb 24, 2015·Journal of the American College of Surgeons·Michael Bouvet, Robert M Hoffman
Oct 4, 2016·Journal of Surgical Oncology·Yukihiko HiroshimaMichael Bouvet
May 17, 2018·Expert Review of Anticancer Therapy·Thinzar M LwinMichael Bouvet
Dec 15, 2020·Surgical Oncology·Hannah M HollandsworthMichael Bouvet
Apr 4, 2021·Journal of Clinical Medicine·Ruth González-GómezAlberto J Schuhmacher
Aug 28, 2021·International Journal of Molecular Sciences·Shiran SuDavid L Brody

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