Comparison of a classical phagocytosis assay and a flow cytometry assay for assessment of the phagocytic capacity of sera from adults vaccinated with a pneumococcal conjugate vaccine

Clinical and Diagnostic Laboratory Immunology
W JansenA Verheul

Abstract

Antibody- and complement-mediated phagocytosis is the main defense mechanism against Streptococcus pneumoniae. A standardized, easy to perform phagocytosis assay for pneumococci would be a great asset for the evaluation of the potential efficacy of (experimental) pneumococcal vaccines. Such an assay could replace the laborious phagocytosis assay of viable pneumococci (classical killing assay). Therefore, a newly developed phagocytosis assay based on flow cytometry (flow assay) was compared with the conventional killing assay and enzyme-linked immunosorbent assay (ELISA), using sera obtained from adults pre- and postvaccination with either a bivalent conjugate, a tetravalent conjugate, or the 23-valent polysaccharide vaccine. Highly significant correlations were observed between flow assay phagocytosis titers, killing assay phagocytosis titers, and ELISA antibody titers for serotype 6B and 23F as well. For serotype 19F, strong correlations were only observed between killing assay and ELISA titers. A potential drawback of the flow assay might be the low sensitivity compared with that of the killing assay. The choice of what assay to use, however, will depend on the objectives of the assay. When speed, easy performance, sample thr...Continue Reading

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Citations

Jun 20, 2002·Trends in Immunology·Gabriel A RabinovichStefano Iacobelli
Mar 14, 2003·The Journal of Infection·Neil French
Feb 9, 2005·Clinical and Diagnostic Laboratory Immunology·Branda T HuSally A Quataert
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·R A FleckM H Nahm
Jul 11, 2003·Clinical and Diagnostic Laboratory Immunology·Kyung Hyo KimMoon H Nahm
Jun 25, 2003·Current Opinion in Infectious Diseases·Julio Casal, David Tarragó
Aug 21, 2010·Journal of Medical Microbiology·Ema PaulovičováSlavomír Bystrický
Mar 9, 2021·Frontiers in Immunology·Elena BoeroKok P M van Kessel

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