Comparison of a novel microaerobic system with three other gas-generating systems for the recovery of Campylobacter species from human faecal samples

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
F J BoltonA D Sails

Abstract

Three commercial gas-generating systems--CampyGen (Oxoid, UK), Oxoid BR56 (Oxoid, UK), and CampyPak Plus (Becton Dickinson, USA)--and the evacuation replacement technique were compared for the recovery of Campylobacter spp. from 500 human faecal samples collected from patients with gastroenteritis. Four hundred fifty faecal samples were tested upon receipt in the laboratory. Fifty faecal samples that had been previously found to be positive for Campylobacter spp. were tested retrospectively; these had been stored at 4 degrees C for more than 48 h. A total of 41 (9.1%) of the fresh faecal samples and 41 of 50 (82%) of the stored faecal samples were positive for thermophilic campylobacters. The CampyGen, the Oxoid BR56, the CampyPak Plus, and the evacuation replacement system detected Campylobacter spp. in 40 (97.6%), 39 (95.1%), 41 (100%), and 41 (100%) of the positive fresh faecal samples and in 37 (90.2%), 40 (97.6%), 39 (95.1%), and 40 (97.6%) of the stored samples, respectively. There was no statistical difference in performance of any of the four gas systems used (p = 0.98; chi-square test). Eighty-six percent of the isolates were Campylobacter jejuni and 14% were Campylobacter coli. Biotyping and phage typing of the isolat...Continue Reading

References

Aug 1, 1979·Journal of Clinical Microbiology·M A Karmali, P C Fleming
Jul 2, 1977·British Medical Journal·M B Skirrow
Jun 1, 1990·Epidemiology and Infection·S M SalamaD N Hutchinson
Apr 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F J BoltonG Parker
Jan 1, 1987·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E Buse
Nov 1, 1985·Journal of Clinical Pathology·C D RibeiroA D Grimshaw
Aug 1, 1984·Journal of Clinical Pathology·D N Hutchinson, F J Bolton
Jul 1, 1983·Journal of Clinical Pathology·F J Bolton, D Coates
Apr 1, 1982·Journal of Clinical Pathology·F J Bolton, L Robertson
Sep 1, 1993·Journal of Clinical Pathology·S T AspinallD N Hutchinson

❮ Previous
Next ❯

Citations

Sep 8, 2010·Applied and Environmental Microbiology·Reha Onur Azizoglu, Sophia Kathariou
Mar 21, 2007·International Journal of Food Microbiology·Tom HumphreyMogens Madsen
Nov 10, 2007·Environmental Microbiology·Geertje van KeulenR Gary Sawers
Mar 16, 2018·The Cochrane Database of Systematic Reviews·Lawrence Mj BestKurinchi Selvan Gurusamy
Jan 18, 2015·Applied and Environmental Microbiology·Jesse P HarrisonCharles S Cockell
Jan 27, 2017·Journal of the Royal Society, Interface·Jesse P HarrisonCharles S Cockell
Dec 3, 1998·Applied and Environmental Microbiology·A ReezalJ G Anderson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.