Comparison of a PCR assay in whole blood and serum specimens for canine brucellosis diagnosis

The Veterinary Record
L B KeidL J Richtzenhain

Abstract

The performance of a serum PCR assay was compared with that of a blood PCR assay for the diagnosis of canine brucellosis caused by Brucella canis in 72 dogs. The dogs were classified into three groups (infected, non-infected and suspected brucellosis) according to the results of blood culture and serological tests. The sensitivities of blood PCR and serum PCR were, respectively, 97.14 per cent and 25.71 per cent. The specificities of both were 100 per cent. In the group of dogs with suspected brucellosis, three were positive by blood PCR and none was positive by serum PCR. Serum PCR showed little value for the direct diagnosis of canine brucellosis as the assay had low diagnostic sensitivity and fewer positive dogs were detected by this test than by blood culture, blood PCR, rapid slide agglutination test (RSAT) and RSAT with 2-mercaptoethanol.

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Citations

Aug 19, 2014·Comparative Immunology, Microbiology and Infectious Diseases·Sung-Il KangMoon Her
Apr 10, 2012·The Veterinary Clinics of North America. Small Animal Practice·Elizabeth M Graham, David J Taylor
Nov 2, 2011·The Veterinary Clinics of North America. Small Animal Practice·Chelsea L Makloski
Apr 7, 2015·Comparative Immunology, Microbiology and Infectious Diseases·Sung-Il KangSuk Chan Jung
Mar 20, 2021·Frontiers in Veterinary Science·Renato L SantosTatiane A Paíxão
May 8, 2021·Journal of Veterinary Internal Medicine·Charlotte NuryJulie Arsenault

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