PMID: 9420136Jan 7, 1998Paper

Comparison of a radioallergosorbent (RAST) inhibition method and a monoclonal enzyme linked immunosorbent assay (ELISA) for aeroallergen measurement

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
A RenströmP Malmberg

Abstract

Mouse and rat urinary proteins are potent occupational allergens for exposed personnel. Methods of measuring airborne allergens differ greatly, and reported levels of allergens vary considerably between laboratories. To compare the values obtained using two different methods of allergen detection. Air samples were collected in rat rooms in Sweden and the United Kingdom at 2 L/min on to polytetrafluoroethylene (PTFE) filters and extracted in buffer containing 0.5% v/v Tween 20. Airborne rat urinary allergen (RUA) was measured in all samples by both RAST inhibition using a polyclonal human serum pool (UK) and a two monoclonal antibody sandwich ELISA employing antibodies specific for Rat n 1.02 (alpha2u-globulin) (Sweden). The two methods gave values which were correlated (r2 log values = 0.72, P<0.0001), but differed by several orders of magnitude (median [range] ratio of RAST inhibition/ELISA = 316 [7-26(80)]. There was a systematic bias: as the absolute values increased, the difference in the measurements increased. The rat urine standards used were antigenically similar. A large contrast in RUA values obtained from the two assays was observed in this study. This may be primarily due to methodological differences, but variation...Continue Reading

Citations

Feb 5, 2000·Occupational and Environmental Medicine·D HeederikM J Nieuwenhuijsen
Aug 5, 2003·Environmental Health Perspectives·Ginger L ChewPatrick L Kinney
May 12, 2012·American Journal of Industrial Medicine·Anna Dahlman-HöglundEva Andersson
Aug 19, 2004·The Journal of Allergy and Clinical Immunology·Elena FerrariMartin D Chapman
Apr 22, 2004·The Journal of Allergy and Clinical Immunology·Anne KorpiAnna-Liisa Pasanen
Oct 29, 2005·Journal of Immunological Methods·Rose NjeminiTony Mets
Apr 20, 2002·Current Opinion in Allergy and Clinical Immunology·D Heederik
Jun 26, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P GriffinA D Curran

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