Comparison of a radioimmunoprecipitation assay to immunoblotting and ELISA for detection of antibody to African swine fever virus

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
C AlcarazJ M Escribano

Abstract

A radioimmunoprecipitation assay (RIPA) has been developed for detection of antibody to African swine fever virus (ASFV) and compared with the immunoblot assay with regard to sensitivity and specificity. Two hundred seven field sera, obtained from pigs in Spain from different geographic areas between 1975 and 1986, that were positive by ASFV enzyme-linked immunosorbent assay (ELISA) were also analysed by immunoblot assay and RIPA. By serum dilution experiments, the RIPA appeared at least as sensitive as the ELISA and immunoblotting tests, although ELISA and RIPA detected antibodies to ASFV earlier in natural infection than did the immunoblot assay, as disclosed by animal inoculation studies. The most antigenic ASFV-induced proteins in natural infection detected by RIPA were the viral proteins p243, p172, p73, p25.5, p15, and p12 and the infection proteins p30 and p23.5. In the immunoblot assay, the proteins that were most reactive with the same sera were the viral protein p25.5 and the infection proteins p30, p25, and p21.5. Only 1 serum, from an animal infected with ASFV, was negative by immunoblot assay but showed a positive result by RIPA. A modification of conventional RIPA was performed using a dot transference of immunopr...Continue Reading

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Citations

Mar 7, 2006·Journal of Clinical Microbiology·Carmina GallardoJose Manuel Sanchez-Vizcaino
Nov 20, 2012·Virus Research·José M EscribanoCovadonga Alonso
Oct 8, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Zhenping ChenPaul J Boor
Oct 9, 2012·Virus Research·Patricia de LeónAngel L Carrascosa
Mar 19, 2016·Journal of Cellular Physiology·Zhuo LiCheng Du
Jul 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·E M Zhou, M K Chan
Jul 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M J PastorJ M Escribano
Dec 1, 2018·The Journal of Comparative Neurology·Kyria M WebsterBridgette D Semple
May 8, 2009·Clinical and Vaccine Immunology : CVI·Carmina GallardoAlexandre Leitão
Aug 10, 2017·American Journal of Physiology. Endocrinology and Metabolism·Lauren E WrightRosario Rizzuto
Apr 10, 2018·Frontiers in Physiology·Esther LópezPedro C Redondo Liberal
Sep 8, 2017·Frontiers in Plant Science·Ascensión Martínez-MárquezRoque Bru-Martínez
Jul 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tongguang WangAvindra Nath
Feb 5, 2021·Scientific Reports·Mikhail Oliveira LeastroJesús A Sánchez-Navarro

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