PMID: 8438903Mar 1, 1993

Comparison of acute myeloid leukemia occurring de novo or preceded by a myelodysplastic stage: differences in cellular DNA content

American Journal of Hematology
S WidellP Reizenstein

Abstract

The DNA content of bone marrow cells in patients with acute leukemia preceded by a myelodysplastic stage (MDS-AML) was compared to that in patients with de novo AML. We studied granulocytes, lymphocytes, monocytes, and blasts/promyelocytes from Feulgen-stained bone marrow smears of 11 patients with de novo AML, ten patients with MDS-AML, and 13 apparently healthy controls. The mean amount of DNA per cell (DNA index; DI) in each cell population was determined using a digital video-based image-analyzing system (CAS-100). Analysis of variance (F test) showed a significant difference in the DNA content between de novo AML on one hand and MDS-AML and controls on the other as regards to blasts/promyelocytes (P < 0.01), lymphocytes (P < 0.05), and monocytes (P < 0.01), respectively. In three of 11 (27%) patients with de novo AML, a lower than normal limit DI was found both in immature and mature bone marrow cells. Patients with MDS-AML had those of DI values similar to normal controls. In consequence, a significantly reduced mean DI was found in patients with de novo AML in blasts/promyelocytes (P < 0.01), and monocytes (P < 0.05) compared to both normal controls and MDS-AML. Together with data published separately, suggesting differe...Continue Reading

References

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Citations

Nov 11, 1999·British Journal of Haematology·S WidellJ M Bennett
Aug 1, 1993·American Journal of Hematology·S WidellP Reizenstein

Related Concepts

Teens
Marrow
Bone Marrow Cells
DNA, Neoplasm
Individuality
Acute Monocytic Leukemia
Hematopoetic Myelodysplasia
Reproducibility of Results
Acute Myeloid Leukemia, M1
Acute Myelomonocytic Leukemia

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