Comparison of agar gel electrophoresis and isoelectric focusing in multiple sclerosis and subacute sclerosing panencephalitis

Annals of Neurology
D H MattsonB G Arnason

Abstract

Isoelectric focusing (IEF) and agar gel electrophoresis (AGE) were used to examine cerebrospinal fluid (CSF) and sera from patients with multiple sclerosis (MS) and subacute sclerosing panencephalitis (SSPE). All 15 SSPE samples and 29 of the 33 MS CSF samples showed oligoclonal IgG bands by AGE and IEF. Serum bands were more frequent in SSPE than in MS and were more commonly detected by IEF than by AGE. In MS CSF the number of bands on IEF correlated with: (1) disease duration, (2) CSF IgG, (3) CSF IgG/albumin ratio, and (4) central nervous system IgG synthesis. Serial studies revealed increases in IEF band number in 4 of 10 MS and 3 of 5 SSPE CSF specimens; fluctuations in band intensity were also noted. Densitometric scans of CSF IEF gels showed high, sharply angled IgG band peaks in SSPE; the CSF band peaks in MS were flatter and had higher background IgG. The SSPE pattern could be made to resemble the MS pattern through addition of normal polyclonal IgG to SSPE CSF. These findings suggest that in addition to oligoclonal IgG, polyclonal IgG is synthesized locally within the central nervous system in MS.

References

Nov 1, 1978·Journal of the Neurological Sciences·A Sidén, K G Kjellin
Nov 1, 1977·Annals of Neurology·K P Johnson, B J Nelson
Oct 1, 1974·Journal of the Neurological Sciences·K G Kjellin, O Vesterberg
Oct 1, 1971·Archives of Neurology·H Link, R Müller
Aug 1, 1968·Journal of Neurochemistry·L BergmannL Svennerholm

❮ Previous
Next ❯

Citations

Jun 1, 1987·Journal of Neurology·F BraccoB Tavolato
Dec 1, 1989·Italian Journal of Neurological Sciences·P GalloL Battistin
Apr 1, 1991·Italian Journal of Neurological Sciences·L M Grimaldi, G V Martino
Oct 1, 1987·Journal of the Neurological Sciences·M Sandberg-WollheimE Norrby
Aug 1, 1983·Journal of Neuroscience Methods·R P Roos, M Lichter
Jan 1, 1981·Clinical Neurology and Neurosurgery·P Delmotte, H Carton
Jan 1, 1986·The Journal of Experimental Medicine·M J Walsh, W W Tourtellotte
Jun 1, 1983·Journal of Neurology, Neurosurgery, and Psychiatry·E J ThompsonP Rudge
May 12, 2006·Neurological Research·Gregory P OwensMark P Burgoon
May 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mark P BurgoonDonald H Gilden
Feb 1, 1983·Journal of Neurology, Neurosurgery, and Psychiatry·E J ThompsonP Rudge
Aug 10, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Helena BatoulisStefanie Kuerten
Feb 1, 1985·Seminars in Arthritis and Rheumatism·J D SmileyL H Paradies
Jan 1, 1984·Annals of the New York Academy of Sciences·J N Whitaker
Apr 7, 2017·Journal of Neurology·Diana FerraroUNKNOWN RIREMS group (Rising Italian Researchers in Multiple Sclerosis)
Jun 1, 1982·Journal of Neuroimmunology·D H MattsonB G Arnason
Oct 8, 1998·Multiple Sclerosis : Clinical and Laboratory Research·P D MehtaA M Rostami
Jan 1, 1991·Critical Reviews in Clinical Laboratory Sciences·P D Mehta
Jan 1, 1983·Annals of Neurology·J R MillerC T Bever
May 1, 1992·The Journal of Dairy Research·E I el AgamyR Assaf
Jun 30, 2004·Multiple Sclerosis : Clinical and Laboratory Research·B N HammackD H Gilden
Nov 1, 1996·Multiple Sclerosis : Clinical and Laboratory Research·D H GildenG P Owens
Jan 1, 1985·Annals of Neurology·W W TourtellotteK Syndulko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.