Comparison of age-related differences in expression of phospholipid hydroperoxide glutathione peroxidase mRNA and activity in various tissues of pigs

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
X G LeiK R Roneker

Abstract

Phospholipid hydroperoxide glutathione peroxidase (PHGPX) is the second identified Se-dependent intracellular glutathione peroxidase (PHGPX) that reduces phospholipid hydroperoxides. The objective of this study was to determine the developmental regulation of PHGPX expression in tissues of neonatal, weanling and finishing pigs (Sus scrofa) compared with the expression of the classic Se-dependent cellular glutathione peroxidase (GPX) and the Se-independent enzyme, glutathione S-transferase (GST). Eight different tissues were collected from Se-adequate male pigs aged 1, 28 and 180 days, and supernatant of the tissue homogenate was assayed for PHGPX, GPX and GST activities by using phosphatidylcholine hydroperoxide, hydrogen peroxide and 1-chloro-2,4-dinitrobenzene as substrate, respectively. Total RNA was isolated from four tissues and assayed for PHGPX mRNA expression. Both mRNA and activity expression of PHGPX in most assayed tissues was increased as pigs became older (P < 0.05), but increases in PHGPX mRNA levels between ages did not fully account for all changes in activity. Expression of GPX activity was increased more than that of PHGPX between day 1 and day 28 (P < 0.0001). Expression of GST activity in various tissues was...Continue Reading

References

Apr 30, 1990·Mechanisms of Ageing and Development·R S SohalU T Brunk
Jan 1, 1987·Biology of the Neonate·T YoshiokaY Takehara
Sep 1, 1986·Journal of Animal Science·J L Gill
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Jul 15, 1994·Biochemical and Biophysical Research Communications·M J ChristensenC D Wray
Jun 1, 1994·Journal of Medical Virology·M A BeckO A Levander

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Citations

Jul 28, 2009·Mechanisms of Ageing and Development·Thorsten PetersKarin Scharffetter-Kochanek
Dec 8, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E ZoidisK Feggeros
Jul 17, 2010·Microbiological Research·Simone PienizAdriano Brandelli
Apr 17, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C L XuJ Feng

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