Comparison of Amplicor, in-house PCR, and conventional culture for detection of Mycobacterium tuberculosis in clinical samples.

Journal of Clinical Microbiology
J SchirmJ G Mulder

Abstract

Five hundred four clinical specimens (337 sputum and 167 bronchial samples) from 340 patients were tested for the presence of M. tuberculosis complex by the Amplicor M. tuberculosis test and by an in-house PCR. The results were compared with those obtained by conventional culture and by direct microscopy. Thirty specimens (from 14 patients) were positive by in-house PCR, 25 (from 13 patients) were positive by the Amplicor M. tuberculosis test, and 24 (from 10 patients) were positive by culture. Cultures from 16 specimens were contaminated with other bacteria. Strong inhibition of in-house PCR was found with three samples. After discordancy analyses, with clinical data as supportive evidence for tuberculosis, 27 true-positive and 458 true-negative samples were defined. On the basis of these figures, the sensitivities of the Amplicor M. tuberculosis test, in-house PCR, culture, and microscopy were 70.4, 92.6, 88.9, and 52.4%, respectively. The specificities of all four tests were higher than 98%. The good performance of the in-house PCR for detection of M. tuberculosis makes it a very useful additional tool in M. tuberculosis diagnostics. In contrast, the Amplicor test needs to be improved. Twenty-three of the Amplicor-negative s...Continue Reading

References

Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Oct 1, 1990·Journal of Clinical Microbiology·U SjöbringH Miörner
Sep 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M T La RoccoE Macias
Dec 1, 1994·The Journal of Hospital Infection·F A DrobniewskiA H Uttley
Dec 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A B AndersenN G Stoker

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Citations

Jan 28, 1998·American Journal of Respiratory and Critical Care Medicine·R A CohenK Kaul
Jun 11, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Martín J ZumárragaAngel Cataldi
Mar 5, 2003·Expert Opinion on Pharmacotherapy·Damian Walker, Warren Stevens
Feb 27, 1999·The Pediatric Infectious Disease Journal·N NeuP Della-Latta
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I C ShamputaF Portaels
Dec 12, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D Gomez-PastranaJ Navarro
Apr 5, 2003·Journal of Medical Microbiology·Ayman Mohamed MareiAfify Fahmy Afify
Oct 21, 1999·Scandinavian Journal of Infectious Diseases·A RothH Mauch
Oct 27, 1999·Pediatric Pulmonology·D Gomez-PastranaJ Navarro
Dec 14, 2007·Tropical Medicine & International Health : TM & IH·S HiraoL E Cuevas
Aug 31, 2012·Journal of Clinical Microbiology·Yukio HidaMasayuki Iwano
Jan 18, 2003·Paediatric Respiratory Reviews·G de Charnace, C Delacourt
Sep 28, 2001·Journal of Clinical Microbiology·B BöddinghausT Bittner
May 23, 1998·Journal of Clinical Microbiology·J MagdalenaC Locht
Dec 17, 1997·Journal of Clinical Microbiology·J P CegielskiL B Reller

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