PMID: 7536370Jan 1, 1995Paper

Comparison of an antigen capture enzyme-linked assay with reverse transcription--polymerase chain reaction and cell culture immunoperoxidase tests for the diagnosis of ruminant pestivirus infections

Veterinary Microbiology
G W HornerT Houghton

Abstract

A study to compare the merits of three different tests for the diagnosis of ruminant pestivirus infections was carried out. Sensitivity studies using reference strains of bovine viral diarrhoea virus (BVDV) and buffy coat samples from persistently infected (PI) carriers showed the reverse transcription-polymerase chain reaction (RT-PCR) had a greater sensitivity than the other tests. The antigen capture enzyme-linked immunosorbent assay (ELISA) was least sensitive and could only be used on samples containing cells (tissue or blood). When 169 clinical samples were examined, the RT-PCR detected the most positives (42) compared to the ELISA (32) and the immunoperoxidase test (IPT) (20). The RT-PCR was more successful when specific antibody was also present in the sample. The lower sensitivity of the IPT was related to the use of a 1 passage (4-day) test and the testing of toxic or contaminated samples. The ELISA was found to be most suitable for large-scale testing for the diagnosis and control of pestivirus infections.

References

Aug 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·E E GottschalkV Moennig
Jan 1, 1992·Archives of Virology·K M Tham, W L Stanislawek
Jan 1, 1991·Veterinary Microbiology·C HertigE Peterhans
Jan 1, 1990·Archives of Virology·B A Schroeder, T C Balassu-Chan
May 18, 1989·Nature·S Kwok, R Higuchi
Apr 1, 1993·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·H AlansariL N Potgieter
Apr 1, 1957·Proceedings of the Society for Experimental Biology and Medicine·N R UNDERDAHLA B HOERLEIN
Sep 1, 1992·New Zealand Veterinary Journal·M X Motha, K M Tham
Sep 1, 1993·New Zealand Veterinary Journal·G W Horner, D M Orr

❮ Previous
Next ❯

Citations

Nov 6, 2003·Journal of Virological Methods·C Letellier, P Kerkhofs
Aug 24, 2010·Journal of Veterinary Internal Medicine·M MudryP Zanolari
Sep 20, 2005·Preventive Veterinary Medicine·C LetellierP Kerkhofs
Dec 9, 2004·The Veterinary Clinics of North America. Exotic Animal Practice·Melissa Kennedy
May 11, 2013·Acta Veterinaria Hungarica·Sibel YavruAyhan Ata
Sep 24, 2013·The Veterinary Journal·Sasha R LanyonJoe Brownlie
Mar 24, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R W RenshawE J Dubovi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.