Comparison of an extender containing defined milk protein fractions with a skim milk-based extender for storage of equine semen at 5 degrees C

Theriogenology
R PaglC Aurich

Abstract

A problem of semen extenders based on milk or egg yolk is the fact that these biological products consist of a variety of substances. Extenders containing only components with clearly protective effects on spermatozoa would thus be an advantage. In this study, we have compared the effects of an extender containing defined caseinates and whey proteins only (EquiPro, defined milk protein extender) with skim milk extender on equine spermatozoa during cooled storage. The defined milk protein extender was used with and without the antioxidant N-acetyl cysteine (NAC). In a second experiment, semen was diluted with PBS or defined milk protein extender and was either stored directly or 90% of seminal plasma was removed by centrifugation and replaced by defined milk protein extender before storage. In both experiments, eight stallions were available for semen collections. Motility, velocity and membrane integrity of spermatozoa were determined by CASA immediately after semen processing and after 24, 48 and 72 h of storage at 5 degrees C. Total motility after 24 h of storage was lowest in semen diluted with PBS (p<0.05 versus all extenders). At 48 and 72 h, motility of spermatozoa in defined milk protein extender was significantly (p<0.0...Continue Reading

References

Jan 1, 1990·Journal of Reproduction and Fertility·R A Harrison, S E Vickers
Aug 6, 2005·Animal Reproduction Science·Christine Aurich

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Citations

Jul 20, 2007·Reproduction in Domestic Animals = Zuchthygiene·C AurichF Müller-Schlösser
Feb 18, 2016·BioMed Research International·Zamira Gibb, Robert J Aitken
Aug 3, 2010·Reproductive Biomedicine Online·J M MorrellA Johannisson
Nov 29, 2007·Reproduction in Domestic Animals = Zuchthygiene·S PriceC Aurich
Apr 25, 2012·Reproduction in Domestic Animals = Zuchthygiene·I Barrier-BattutM Vidament
Mar 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·M Mata-CampuzanoF Martínez-Pastor
Oct 25, 2011·Reproduction in Domestic Animals = Zuchthygiene·A K SinghS K Atreja
Apr 24, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Zhao NamulaTakeshige Otoi
Jan 5, 2011·Theriogenology·Sabine HeckenbichlerChristine Aurich
Jun 1, 2016·Theriogenology·Christine AurichDragos Scarlet
Mar 2, 2013·Equine Veterinary Journal. Supplement·C J DeanD D Varner
Dec 24, 2019·Reproduction in Domestic Animals = Zuchthygiene·María José ContrerasRicardo Felmer
Dec 14, 2011·Equine Veterinary Journal·M K HoogewijsA van Soom
Jul 25, 2019·Animals : an Open Access Journal From MDPI·Alicja KowalczykMarian Kuczaj
May 23, 2012·Reproduction in Domestic Animals = Zuchthygiene·D ScarletC Aurich
Dec 29, 2018·Reproduction in Domestic Animals = Zuchthygiene·Tammo BussChristine Aurich
Dec 9, 2020·Animals : an Open Access Journal From MDPI·Islam M SaadeldinSeok Hee Lee
Mar 6, 2021·Journal of Equine Veterinary Science·Johanna PapinJane M Morrell
Sep 26, 2020·Journal of Equine Veterinary Science·Gabriela A CamposJosé Antonio Dell'Aqua

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