Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis

Indian Journal of Medical Microbiology
S ShekatkarS C Parija

Abstract

The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira by culture has a low sensitivity and the microscopic agglutination test (MAT) is time consuming To overcome these problems, a rapid latex agglutination test (LAT) has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis. We compared the efficiency of the LAT to a commercially available IgM ELISA and MAT. A total of 150 serum samples were tested by LAT, IgM ELISA, and MAT. The positivity was 26.7%, 26% and 24% respectively. The sensitivity and specificity of LAT as compared to MAT was 90.62 and 91.96% respectively. Even though LAT and ELISA showed similar results, its rapidity and simplicity made latex agglutination test more suitable as a rapid screening test.

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Citations

Sep 4, 2014·Asian Pacific Journal of Tropical Biomedicine·Chintana ChirathawornDuangjai Suwancharoen
Jul 5, 2013·PLoS Neglected Tropical Diseases·Cyrille GoarantSuzanne Chanteau
Jul 14, 2018·Expert Review of Proteomics·Arada Vinaiphat, Visith Thongboonkerd
Dec 22, 2017·Ciência & saúde coletiva·Maria Ines RosaFernanda Oliveira Meller
Nov 28, 2012·Indian Journal of Medical Microbiology·A KannanS R Rathinam

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