Comparison of analytical protein separation characteristics for three amine-based capillary-channeled polymer (C-CP) stationary phases

Analytical and Bioanalytical Chemistry
Liuwei Jiang, R Kenneth Marcus

Abstract

Capillary-channeled polymer (C-CP) fiber stationary phases are finding utility in the realms of protein analytics as well as downstream processing. We have recently described the modification of poly(ethylene terephthalate) (PET) C-CP fibers to affect amine-rich phases for the weak anion-exchange (WAX) separation of proteins. Polyethylenimine (PEI) is covalently coupled to the PET surface, with subsequent cross-linking imparted by treatment with 1,4-butanediol diglycidyl ether (BUDGE). These modifications yield vastly improved dynamic binding capacities over the unmodified fibers. We have also previously employed native (unmodified) nylon 6 C-CP fibers as weak anion/cation-exchange (mixed-mode) and hydrophobic interaction chromatography (HIC) phases for protein separations. Polyamide, nylon 6, consists of amide groups along the polymer backbone, with primary amines and carboxylic acid end groups. The analytical separation characteristics of these three amine-based C-CP fiber phases are compared here. Each of the C-CP fiber columns in this study was shown to be able to separate a bovine serum albumin/hemoglobin/lysozyme mixture at high mobile phase linear velocity (∼70 mm s(-1)) but with different elution characteristics. These ...Continue Reading

References

Sep 19, 1998·Journal of Chromatography. a·S C Goheen, J L Hilsenbeck
Oct 28, 1999·Journal of Chromatography. a·K Miyabe, G Guiochon
Aug 21, 2001·Analytical Chemistry·N TanakaN Ishizuka
Jun 13, 2002·Journal of Chromatography. a·Hanfa ZouQuanzhou Luo
Feb 15, 2003·Journal of Chromatography. a·R Kenneth MarcusJ David Jenkins
Nov 5, 2003·Journal of Chromatographic Science·Dwella K Nelson, R Kenneth Marcus
Oct 1, 2004·Analytical Chemistry·Peter DePhillipsAbraham M Lenhoff
Jan 12, 2005·Journal of Chromatography. a·Guo-Yong SunYan Sun
Nov 12, 2005·Analytical and Bioanalytical Chemistry·Rayman D StanelleR Kenneth Marcus
Apr 30, 2008·Biotechnology Progress·Uwe Gottschalk
Jun 28, 2012·Analytical and Bioanalytical Chemistry·K Manoj Randunu, R Kenneth Marcus
Oct 8, 2013·Journal of Chromatography. a·Stephanie A SchusterJoseph J Kirkland
Jan 1, 2014·Journal of Separation Science·Abby J Schadock-Hewitt, R Kenneth Marcus
Jan 31, 2015·Nature Reviews. Drug Discovery·Asher Mullard
Oct 1, 2015·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Gustavo Grampp, Thomas Felix

❮ Previous
Next ❯

Citations

Nov 15, 2018·Journal of Separation Science·David SýkoraVladimír Král

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.