Comparison of antigenic sites of subtype-specific respiratory syncytial virus attachment proteins.

The Journal of General Virology
Edward WalshP R Paradiso

Abstract

A panel of 19 monoclonal antibodies (MAbs) were used to probe the antigenic relationships between the G (attachment) proteins of A and B respiratory syncytial virus (RSV) subtypes (GA and GB). At least three and two antigenic sites were present on GA and GB, respectively, including a shared neutralizing site. Most of the antibodies had some degree of complement-independent neutralizing capacity, but in common was a large neutralization-resistant fraction of virus (range 13 to 78%). Passive administration of MAbs to the cross-reactive antigenic site reduced pulmonary virus titres of both A and B subtype virus in the cotton rat model. Protection with subtype-specific MAbs, however, did not always correlate with in vitro neutralizing capacity. The cross-reactive antigenic site appears to be stable to denaturation by polyacrylamide gel electrophoresis and is present on the unglycosylated and partially glycosylated forms of GA and GB by Western blot analysis of infected cell lysates.

Citations

Jan 1, 1990·Archives of Virology·T G Kimman, F Westenbrink
Mar 17, 2010·Cellular & Molecular Immunology·Qigui YuXuebin Qin
Sep 30, 2010·Immunotherapy·Lawrence M KauvarRalph A Tripp
Feb 26, 2016·Journal of the Pediatric Infectious Diseases Society·Geoffrey A Weinberg
Oct 1, 1991·FEMS Microbiology Immunology·G L Toms
Feb 26, 2020·Expert Opinion on Investigational Drugs·Harrison C Bergeron, Ralph A Tripp
Oct 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ellen J CollariniLawrence M Kauvar
Apr 7, 2017·PloS One·Jeong-Yoon Lee, Jun Chang
Mar 20, 2020·Science Translational Medicine·Simon B DrysdaleCharles J Sande
May 1, 1993·Journal of Clinical Microbiology·W M SullenderL J Anderson

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