Comparison of Aqueous and Vitreous Lymphocyte Populations From Two Rat Models of Experimental Uveitis

Investigative Ophthalmology & Visual Science
Kathryn L PeppleRussell N Van Gelder

Abstract

To compare lymphocyte populations present within inflamed eyes in two rat models of autoimmune uveitis. Experimental autoimmune uveitis (EAU) and primed mycobacterial uveitis (PMU) were initiated in Lewis rats. Aqueous and vitreous were collected at peak inflammation (PMU at day 2, EAU at day 14). The number of cells in the aqueous and vitreous was determined and compared for each eye and between the two models. Intraocular CD-19+ B cells, CD3+ T cells, and CD4+ or CD8+ T-cell subpopulations were identified by flow cytometry and compared between EAU and PMU. The median number of cells/mL collected from PMU aqueous (7.98 × 107 cells/mL), was not significantly different from the number of cells collected from EAU aqueous (1.61 × 107 cells/mL, P = 0.94). EAU aqueous contains a significantly larger mononuclear population (median 61%, interquartile range [IQR] 44%-67%) than PMU (median 9%, IQR 8%-10% [P < 0.0001]). Within the mononuclear population, EAU and PMU aqueous demonstrate similar proportions of CD3+, CD4+ T cells. However, EAU has a larger CD3+, CD8+, T-cell population than PMU, and this population also demonstrates co-expression of CD45R. B cells comprise a significantly larger median percentage of cells in EAU aqueous (me...Continue Reading

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Citations

May 20, 2020·The British Journal of Ophthalmology·Ester CarreñoNicolas Alejandre
Jan 26, 2021·Frontiers in Immunology·Roxane L Degroote, Cornelia A Deeg
Sep 2, 2021·Ocular Immunology and Inflammation·Jike SongHongsheng Bi
Dec 8, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·JodiRae DeDreuA Sue Menko

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Methods Mentioned

BETA
flow cytometry
FACS
light scattering

Software Mentioned

GraphPad
Prism
FlowJo

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