PMID: 8595856Jan 15, 1996Paper

Comparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection

FEMS Microbiology Letters
E K BlackM Belosevic

Abstract

In vitro excystation, vital dyes (4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI), and infectivity in neonatal CD-1 mice were used to assess the viability of Cryptosporidium parvum oocysts after chemical disinfection. In vitro excystation and DAPI/PI staining provided similar estimates of viability in bench-scale experiments, but both of these methods significantly overestimated the viability when compared with infectivity (Pr < or = 0.01). Infectivity was the most reliable measure of the viability of C. parvum oocysts following chemical disinfection.

References

Nov 1, 1987·The Journal of protozoology·D B Woodmansee
Nov 1, 1993·Applied and Environmental Microbiology·G R FinchM Belosevic

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Citations

Jul 2, 2011·Environmental Science and Pollution Research International·María-Eugenia RubianoFrancisco Lucena
Sep 15, 2009·Applied and Environmental Microbiology·Cristin C BresciaEric N Villegas
Aug 2, 2011·Applied and Environmental Microbiology·Zhanbei Liang, Ann Keeley
Feb 2, 2010·Applied and Environmental Microbiology·George Bajszár, Alexander Dekonenko
Jun 19, 2012·Applied and Environmental Microbiology·Heidi H PetersenAnders Dalsgaard
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